CONTEXT We have recently shown that the tet family enzymes convert 5-™ 5-hydroxymethylcytosin (5-HMC) to DNA. 5-HMC is present at high levels in embryonic stemRead More
We have developed a general and simple method for directing specific sequence modifications in a plasmid using the amplification approved by the reaction of theRead More
Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR.
Viral hemorrhagic fevers (VHFS) are acute infections with high mortality rates. Significant VHF agents are Ebola and Marburg viruses (MBGV / EBOV), Lassa Virus (LASV),Read More
A polymerase thermostable DNA which has an associated exonuclease activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus Furiosus (PFU). To test its loyalty, weRead More
A set of universal oligonucleotide primers for the retained regions of the eubactterial gene 16S arrnes has been designed for use with the 7700 Real-TimeRead More
chemical modifications to DNA, such as modification 2 ‘, is expected to increase the utility of DNA biotechnology; However, other forms of modification of DNARead More
Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.
BACKGROUND: Detection of bacteria by PCR applied for screening blood and blood products with special attention platelet concentrates. For practical use is expected that theRead More
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.
DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as anRead More
Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.
Merging AuNPs to polymerase chain reaction (PCR) has become a promising strategy to develop a sensitive sensing platform, since the desired optical properties of AuNPsRead More
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleicRead More