Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

Merging AuNPs to polymerase chain reaction (PCR) has become a promising strategy to develop a sensitive sensing platform, since the desired optical properties of AuNPs and the power of exponential PCR amplification. However, the combination of AuNPs for PCR typically fail to achieve the expected sensitivity along with additional measures. Here we report a single step and universal PCR-based biosensor for detection depending on the size of nucleic acids and proteins by using the technique of dynamic light scattering (DLS).

The sensing system employs a modified DNA AuNPs (AuNPs-DNA) to serve as a TaqMan probe as the probe signal, in which the DNA on the surface of AuNPs can be parsed by Taq polymerase during PCR extension, causing aggregation of AuNPs to be detected by DLS. This strategy allows the recognition of the target-specific sequences of double-stranded DNA (dsDNA), cope with changes in background signal that was triggered by the increase of PCR cycles. Further demonstrating its application in the detection of foodborne pathogens Listeria monocytogenes with a detection limit of 1.2 fg uL-1, which is more sensitive than colorimetric methods.

In addition, by utilizing the proximity test strategy, this biosensor demonstrated the ability to detect homogeneous proteins, which indicates the detection limits for the model thrombin 13:00. Together, the work shows a reliable strategy to combine AuNPs in the PCR amplification process as the probe signal, rather than a process of post-PCR.

Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.
Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

recombinant novel properties Sso7d-Taq DNA polymerase purified using a two-phase extraction of water: The utility of the enzyme in the diagnosis of the virus.

Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification of novel methodologies Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.

The fusion enzyme usefulness in carrying out human gene PCR directly from whole blood and in the detection of hepatitis B virus from clinical samples is shown in this article. We present data on improved thermo-stability of the S-Taq DNA polymerase over Taq DNA polymerase and also provide evidence of high stability with detergent in comparison with Taq polymerase. S-protein purified Taq shows the acceptable limit of the level of the host genomic DNA without the use of DNA accelerate DNases and other agents and show promising potential for use in diagnostic PCR-based, in-situ PCR and forensic science.


To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine. The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed.

DNA-fectamine

DNAF001 1ml
EUR 278.81

DNA-Fect

DF37100-1 1 ml
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DNA-Fect293

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T7 DNA

310-005 200µg
EUR 82

T7 DNA

310-025 5x200 µg
EUR 225

DNA Antibody

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DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

DNA antibody

10R-D114b 100 ug
EUR 560
Description: Mouse monoclonal DNA antibody

DNA pol

ABF9055 100 ug
EUR 438

DNA antibody

10-D20A 500 ug
EUR 493
Description: Mouse monoclonal DNA antibody

DNA antibody

10C-CR6034M1 100 ug
EUR 241
Description: Mouse monoclonal DNA antibody

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
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Mouse Anti-DNA Antibody (DNA) ELISA Kit

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Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

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DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

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DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

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Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-YP889065HU
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  • EUR 1442.00
  • EUR 382.00
  • 100ug
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-BP889065HU
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  • EUR 1135.00
  • EUR 1579.00
  • EUR 470.00
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-EP889065HU
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  • EUR 335.00
  • 100ug
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Maximo DFS-Plus Taq DNA Polymerase DNA-free

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EUR 50

Maximo DFS-Plus Taq DNA Polymerase DNA-free

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Maximo DFS-Plus Taq DNA Polymerase DNA-free

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Recombinant human DNA-directed DNA/RNA polymerase mu

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Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

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Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated

AD-155-B Custom Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled

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Taq DNA Polymerase

BA00103 500U
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Description: High quality Taq polymerase for different PCR variations and downstream applications.

EZ-DNA Reagents

SK8201 100preps
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Bst DNA Polymerase

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Green-DNA Dye

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pUC 18 DNA

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pUC 19 DNA

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pUC 57 DNA

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DNA Concentrator Kit

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EUR 253
Description: Ask the seller for details

Calf thymus DNA

HY-109517 100mg
EUR 1910

DNA Sample Diluent

D006-100 100 ml
EUR 373
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-1000 1000 ml
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-500 500 ml
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Precipitation Buffer

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Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur.

Human DNA Concentrate

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Description: Human DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

E.coli DNA Concentrate

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CHO DNA Concentrate

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CHO DNA Concentrate

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Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

DNA polymerase ? (Rat)

10-101 20ug
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DNA polymerase ? (Rat)

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DNA Pure Kit

FYG601-100P 100 Preps Ask for price

DNA Pure Kit

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BIOTAQ DNA Polymerase

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IMMOLASE DNA Polymerase

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ACCUZYME DNA Polymerase

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Human Genomic DNA

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100bp DNA Marker

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100bp DNA Marker

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Human Genomic DNA

X11000 0.2 ml Ask for price
Description: reagents widely cited

DNA-PK Antibody

33358-100ul 100ul
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DNA-PK Antibody

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Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

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RedTaq DNA Polymerase

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Pfu DNA Polymerase

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Pfu DNA Polymerase

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Description: High quality Pfu polymerase for different PCR variations and downstream applications.

ClearSight DNA Stain

BH40501 1ml
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Description: For DNA staining and vizualization.

Taq DNA polymerase

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

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HotTaq DNA polymerase

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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

100bp DNA ladder

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EUR 217.48

DNA ligase Antibody

20-abx110554
  • EUR 411.00
  • EUR 1845.00
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  • EUR 182.00
  • EUR 300.00
  • 100 ug
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EZ-DNA Reagents

BS8202 100preps
EUR 88.06

2K DNA Marker

20-abx098045
  • EUR 258.00
  • EUR 189.00
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5K DNA Marker

20-abx098048
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15K DNA Marker

20-abx098049
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20-abx098050
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100bp DNA Marker

20-abx098052
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Genomic DNA Kit

20-abx098076
  • EUR 439.00
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  • 200 rxns
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T4 DNA Ligase

abx098136-10kU 10 kU
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T4 DNA Ligase

abx098136-20Ku 20 Ku
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DNA Loading Buffer

abx098143-5ml 5 ml
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Circulating DNA Kit

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Phage λ-DNA

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EUR 102

Phage λ-DNA

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EUR 355

T4 DNA ligase

402-002 400 WEISS UNITS
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T4 DNA ligase

402-010 2000 WEISS UNITS
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DNA PK Antibody

abx149863-100ug 100 ug
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Intercalated DNA Antibody

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DNA Assay Kit

6023 1 kit
EUR 180.5
Description: DNA Assay Kit

Uracil DNA Glycosylase

7-03778 2,000U Ask for price

Uracil DNA Glycosylase

7-03779 10,000U Ask for price

Uracil DNA Glycosylase

7-03780 20,000U Ask for price

Circulating DNA Kit

abx298027-50rxns 50 rxns
EUR 398

Kinetoplast DNA (catenated)

TG2013-1 25 ug
EUR 268

Kinetoplast DNA (catenated)

TG2013-2 50 ug
EUR 380

Kinetoplast DNA (catenated)

TG2013-3 250 ug
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DNA PKcs Antibody

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DNA PKcs Antibody

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Uracil DNA Glycosilase

RP-1470 2000 IU
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Sapphire Baculovirus DNA

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DNA PK Antibody

AF5340 200ul
EUR 304
Description: DNA PK Antibody detects endogenous levels of total DNA PK.

DNA- PK Antibody

ABF0550 100 ug
EUR 438

DNA PK Antibody

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DNA Loading Dye

G030 6X; 3 x 1.0 ml
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The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.