Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

Merging AuNPs to polymerase chain reaction (PCR) has become a promising strategy to develop a sensitive sensing platform, since the desired optical properties of AuNPs and the power of exponential PCR amplification. However, the combination of AuNPs for PCR typically fail to achieve the expected sensitivity along with additional measures. Here we report a single step and universal PCR-based biosensor for detection depending on the size of nucleic acids and proteins by using the technique of dynamic light scattering (DLS).

The sensing system employs a modified DNA AuNPs (AuNPs-DNA) to serve as a TaqMan probe as the probe signal, in which the DNA on the surface of AuNPs can be parsed by Taq polymerase during PCR extension, causing aggregation of AuNPs to be detected by DLS. This strategy allows the recognition of the target-specific sequences of double-stranded DNA (dsDNA), cope with changes in background signal that was triggered by the increase of PCR cycles. Further demonstrating its application in the detection of foodborne pathogens Listeria monocytogenes with a detection limit of 1.2 fg uL-1, which is more sensitive than colorimetric methods.

In addition, by utilizing the proximity test strategy, this biosensor demonstrated the ability to detect homogeneous proteins, which indicates the detection limits for the model thrombin 13:00. Together, the work shows a reliable strategy to combine AuNPs in the PCR amplification process as the probe signal, rather than a process of post-PCR.

Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.
Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

recombinant novel properties Sso7d-Taq DNA polymerase purified using a two-phase extraction of water: The utility of the enzyme in the diagnosis of the virus.

Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification of novel methodologies Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.

The fusion enzyme usefulness in carrying out human gene PCR directly from whole blood and in the detection of hepatitis B virus from clinical samples is shown in this article. We present data on improved thermo-stability of the S-Taq DNA polymerase over Taq DNA polymerase and also provide evidence of high stability with detergent in comparison with Taq polymerase. S-protein purified Taq shows the acceptable limit of the level of the host genomic DNA without the use of DNA accelerate DNases and other agents and show promising potential for use in diagnostic PCR-based, in-situ PCR and forensic science.


To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine. The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed.

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1830

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
EUR 577.2

pUC18 DNA

G40-300 50µg
EUR 67.2

pUC18 DNA

G40-305 5x50µg
EUR 176.4

DNA-Fect

DF37100-1 1 ml
EUR 418.8

pBR322 DNA

MSD14 50ug
EUR 183.19

DNA antibody

10R-D114b 100 ug
EUR 672
Description: Mouse monoclonal DNA antibody

DNA antibody

10C-CR6034M1 100 ug
EUR 289.2
Description: Mouse monoclonal DNA antibody

DNA antibody

10-D20A 500 ug
EUR 591.6
Description: Mouse monoclonal DNA antibody

DNA Antibody

abx023805-02mg 0.2 mg
EUR 777.6

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Activated DNA

80605 500 µL
EUR 125
Description: Activated DNA for PARP assay, for use with BPS's assay kits for PARPs 1, 2, 3, and 6. PARP1 Chemiluminescent Assay Kit, #80551 ; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553; PARP6 Chemiluminescent Assay Kit, #80556; PARP2 Homogeneous Assay Kit, #80702

DNA-Fect293

DF37293-1 1 ml
EUR 349.2

DNA Polymerase

ABF5235 100 ug
EUR 525.6

DNA Polymerase

ABD3082 100 ug
EUR 525.6

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)

TG2000G-7KIT 2000 assays
EUR 3412.8

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)

TG2000G-5KIT 1000 assays
EUR 1934.4

DNA-fectamine

DNAF001 1ml
EUR 334.57

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)

TG2000G-1KIT 100 assays
EUR 456

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)

TG2000G-3KIT 500 assays
EUR 1182

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-BP889065HU
  • EUR 1008.00
  • EUR 398.40
  • EUR 2604.00
  • EUR 1362.00
  • EUR 1894.80
  • EUR 564.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-YP889065HU
  • EUR 703.20
  • EUR 358.80
  • EUR 2606.40
  • EUR 1080.00
  • EUR 1730.40
  • EUR 458.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-EP889065HU
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

pUC 18 DNA

MSD15 50ug
EUR 146.65

pUC 19 DNA

MSD16 50ug
EUR 136.21

pUC 57 DNA

MSD17 50ug
EUR 136.21

Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled

AD-155-U Custom Ask for price

Recombinant human DNA-directed DNA/RNA polymerase mu

P2815 100ug Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

100 bp DNA Ladder

M1158-500 each
EUR 333.6

100 bp DNA Ladder

MD104-01 250 μl
EUR 132

100 bp DNA Ladder

MD104-02 500 μl
EUR 139.2

DNA Pure Kit

FYG601-100P 100 Preps Ask for price

DNA Pure Kit

FYG601-300P 300 Preps Ask for price

Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated

AD-155-B Custom Ask for price

DNA Assay Kit

6023 1 kit
EUR 216.6
Description: DNA Assay Kit

2K DNA Marker

20-abx098045
  • EUR 309.60
  • EUR 226.80
  • 2.5 ml
  • 500 ul

5K DNA Marker

20-abx098048
  • EUR 309.60
  • EUR 226.80
  • 2.5 ml
  • 500 ul

T4 DNA Ligase

abx098136-10kU 10 kU
EUR 385.2

T4 DNA Ligase

abx098136-20Ku 20 Ku
EUR 477.6

Phage λ-DNA

301-025 500 µg
EUR 122.4

Phage λ-DNA

301-100 5x500µg
EUR 426

T4 DNA ligase

402-002 400 WEISS UNITS
EUR 75.6

T4 DNA ligase

402-010 2000 WEISS UNITS
EUR 184.8

DNA Assay Kit

Z5030023 250 assays
EUR 636

15K DNA Marker

20-abx098049
  • EUR 326.40
  • EUR 226.80
  • 2.5 ml
  • 500 ul

1Kb DNA Marker

20-abx098050
  • EUR 309.60
  • EUR 226.80
  • 2.5 ml
  • 500 ul

1Kb DNA Marker

D1101-050 50㎍
EUR 157.2

1Kb DNA Marker

D1101-250 5X50㎍
EUR 360

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

L1007-5 5 mg/well
EUR 7172.4
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.

DNA- PK Antibody

ABF0550 100 ug
EUR 525.6

DNA PK Antibody

ABF5340 100 ug
EUR 525.6

Genomic DNA Kit

20-abx098076
  • EUR 526.80
  • EUR 292.80
  • 200 rxns
  • 50 rxns

DNA PK antibody

20R-1466 100 ug
EUR 807.6
Description: Rabbit polyclonal DNA PK antibody

DNA PK Antibody

AF5340 200ul
EUR 420

DNA PK Antibody

abx149863-100ug 100 ug
EUR 526.8

Calf thymus DNA

HY-109517 100mg
EUR 2292

DNA Loading Dye

G030 6X; 3 x 1.0 ml
EUR 94.8

100bp DNA ladder

9K-004-0002 0.5ml
EUR 260.98

100bp DNA Marker

20-abx098052
  • EUR 326.40
  • EUR 226.80
  • 2.5 ml
  • 500 ul

100bp DNA Marker

D1100-050 50㎍
EUR 198

100bp DNA Marker

D1100-250 5X50㎍
EUR 660

Mouse Anti-DNA Antibody (DNA) ELISA Kit

abx257816-96tests 96 tests
EUR 895.2

DNA PKcs Antibody

48923-100ul 100ul
EUR 399.6

DNA PKcs Antibody

48923-50ul 50ul
EUR 286.8

DNA PKcs antibody

10R-10729 100 ug
EUR 457.2
Description: Mouse monoclonal DNA PKcs antibody

DNA PKcs antibody

70R-50261 100 ul
EUR 292.8
Description: Purified Polyclonal DNA PKcs antibody

DNA-PK Antibody

AF0550 200ul
EUR 420

DNA PKcs antibody

20R-2017 50 ug
EUR 337.2
Description: Rabbit polyclonal DNA PKcs antibody

DNA PKcs antibody

20R-2346 50 ug
EUR 337.2
Description: Rabbit polyclonal DNA PKcs antibody

DNA-PK Antibody

3130-100 each
EUR 405.6

DNA-PK Antibody

33358-100ul 100ul
EUR 302.4

DNA-PK Antibody

33358-50ul 50ul
EUR 224.4

DNA-PK Antibody

AF6631 100ul
EUR 420

Human Genomic DNA

BIO-35025 500µl @ 200ng/µl Ask for price

T4 DNA Polymerase

M1211-100 each
EUR 385.2

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

DL5000 DNA Marker

MD102-01 250 μl
EUR 128.4

DL5000 DNA Marker

MD102-02 500 μl
EUR 135.6

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

PFU DNA Polymerase

9003-2500 each
EUR 614.4

PFU DNA Polymerase

9003-500 each
EUR 216

Laq? DNA Polymerase

9004-2500 each
EUR 732

Laq? DNA Polymerase

9004-500 each
EUR 261.6

DNA Loading Buffer

abx098143-5ml 5 ml
EUR 210

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

DNA Sample Diluent

D006-100 100 ml
EUR 447.6
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-1000 1000 ml
EUR 1400.4
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-500 500 ml
EUR 871.2
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Cleared Plasma

DCP100A-1 100 ml
EUR 417

Taq DNA Polymerase

L7051001 1000 unit
EUR 159

Taq DNA Polymerase

L7051200 200 unit
EUR 41

Mycoplasma DNA Kit

K1416-250 each
EUR 673.2

Mycoplasma DNA Kit

K1416-50 each
EUR 379.2

DL15000 DNA Marker

MD103-01 250 μl
EUR 130.8

DL15000 DNA Marker

MD103-02 500 μl
EUR 138

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

Human Neutrophil Elastase (DNA I) , DNA Aptamer, FITC labeled

AD-155-F Custom Ask for price

Circulating DNA Kit

abx098870-50rxns 50 rxns
EUR 477.6

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

DNA ligase Antibody

20-abx110554
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Circulating DNA Kit

abx298027-50rxns 50 rxns
EUR 477.6

CHO DNA Concentrate

D552 1 ml
EUR 650.4
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

CHO DNA Concentrate

D556 120 ul
EUR 562.8
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

DNA-PKcs Antibody

R31076 100 ug
EUR 356.15
Description: Protein Kinase DNA-Activated catalytic subunit, also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al.(1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller(1992) noted that DNA-PKcs had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al.(1999) demonstrated that the DNA-PKcs protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.

ClearSight DNA Stain

BH40501 1ml
EUR 123.6
Description: For DNA staining and vizualization.

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

E.coli DNA Concentrate

D412 1 ml
EUR 650.4
Description: E.coli DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur.

DNA Storage Solution

LB3401010 10 ml
EUR 34

Ready? DNA Polymerase

M1146-1000 each
EUR 398.4

Ready? DNA Polymerase

M1146-10000 each
EUR 1279.2

Ready? DNA Polymerase

M1146-5000 each
EUR 960

The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.