Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

Merging AuNPs to polymerase chain reaction (PCR) has become a promising strategy to develop a sensitive sensing platform, since the desired optical properties of AuNPs and the power of exponential PCR amplification. However, the combination of AuNPs for PCR typically fail to achieve the expected sensitivity along with additional measures. Here we report a single step and universal PCR-based biosensor for detection depending on the size of nucleic acids and proteins by using the technique of dynamic light scattering (DLS).

The sensing system employs a modified DNA AuNPs (AuNPs-DNA) to serve as a TaqMan probe as the probe signal, in which the DNA on the surface of AuNPs can be parsed by Taq polymerase during PCR extension, causing aggregation of AuNPs to be detected by DLS. This strategy allows the recognition of the target-specific sequences of double-stranded DNA (dsDNA), cope with changes in background signal that was triggered by the increase of PCR cycles. Further demonstrating its application in the detection of foodborne pathogens Listeria monocytogenes with a detection limit of 1.2 fg uL-1, which is more sensitive than colorimetric methods.

In addition, by utilizing the proximity test strategy, this biosensor demonstrated the ability to detect homogeneous proteins, which indicates the detection limits for the model thrombin 13:00. Together, the work shows a reliable strategy to combine AuNPs in the PCR amplification process as the probe signal, rather than a process of post-PCR.

Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.
Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

recombinant novel properties Sso7d-Taq DNA polymerase purified using a two-phase extraction of water: The utility of the enzyme in the diagnosis of the virus.

Using Sso7d from Sulfolobus solfataricus as DNA binding protein fusion Taq DNA polymerase at the amino end, we reported hyper-expression and purification of novel methodologies Sso7d-Taq polymerase (S-Taq) using a two-phase system of water extraction followed by Ni-affinity chromatography.

The fusion enzyme usefulness in carrying out human gene PCR directly from whole blood and in the detection of hepatitis B virus from clinical samples is shown in this article. We present data on improved thermo-stability of the S-Taq DNA polymerase over Taq DNA polymerase and also provide evidence of high stability with detergent in comparison with Taq polymerase. S-protein purified Taq shows the acceptable limit of the level of the host genomic DNA without the use of DNA accelerate DNases and other agents and show promising potential for use in diagnostic PCR-based, in-situ PCR and forensic science.


To develop the structural modifications dNTP compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dyes of different analog responsible and charge distribution throughout the fluorophore. Cy3- derivatives and commercial dUTP and Cy5-dUTP studied in Taq polymerase dependent polymerase chain reaction (PCR) and the primer extension reaction using a model template that contains one, two and three adjacent nucleotides adenine. The relative amount of DNA is amplified and kinetic parameters Km and Vmax characterize grief labeled incorporation have been estimated using fluorescence measurements and analyzed.

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1830

DNA pol

ABF9055 100 ug
EUR 525.6

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
EUR 577.2

T4 DNA Ligase T4 DNA Ligase catalyses

MBT091-5000U 1 unit
EUR 167.61
Description: T4 DNA Ligase T4 DNA Ligase catalyses

DNA PKcs

E8ET1610-12 100ul
EUR 275
Description: Available in various conjugation types.

DNA STAT

TL-4210 100ml
EUR 158

DNA STAT

TL-4220 200ml
EUR 276

pUC18 DNA

G40-300 50µg
EUR 67.2

pUC18 DNA

G40-305 5x50µg
EUR 176.4

DNA-Fect

DF37100-1 1 ml
EUR 418.8

pBR322 DNA

MSD14 50ug
EUR 183.19

Lambda DNA

PCR-259 2 x 1ml
EUR 59.1

DNA Primase

E8ER1907-59 100ul
EUR 275
Description: Available in various conjugation types.

DNA Antibody

abx023805-02mg 0.2 mg
EUR 777.6

DNA antibody

10-D20A 500 ug
EUR 399
Description: Mouse monoclonal DNA antibody

DNA antibody

10R-D114b 100 ug
EUR 517
Description: Mouse monoclonal DNA antibody

DNA antibody

10C-CR6034M1 100 ug
EUR 95
Description: Mouse monoclonal DNA antibody

DNA, Antibody

GWB-29C3BF 1 mg Ask for price

DNA Antibody

GWB-Q01107 0.1 mg Ask for price

DNA Antibody

GWB-7F5457 0.2 mg Ask for price

DNA, Antibody

GWB-76482C 0.1 mg Ask for price

DNA, Antibody

GWB-FA8229 1 mg Ask for price

DNA, Antibody

GWB-B2ACF7 0.1 mg Ask for price

DNA Antibody

GWB-BA4954 0.05 mg Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

DNA-Fect293

DF37293-1 1 ml
EUR 349.2

Activated DNA

80605 500 µL
EUR 125
Description: Activated DNA for PARP assay, for use with BPS's assay kits for PARPs 1, 2, 3, and 6. PARP1 Chemiluminescent Assay Kit, #80551 ; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553; PARP6 Chemiluminescent Assay Kit, #80556; PARP2 Homogeneous Assay Kit, #80702

DiscoveryProbe™ DNA Damage/DNA Repair Library

L1033-.096 100uL/well(10 mM solution),96 Well Deep Well Plate
EUR 5584
Description: A unique collection of 481 DNA Damage/DNA repair-related compounds for DNA damage/DNA repair research.

DiscoveryProbe™ DNA Damage/DNA Repair Library

L1033-.1 100 uL/well(10 mM solution)
EUR 6976
Description: A unique collection of 481 DNA Damage/DNA repair-related compounds for DNA damage/DNA repair research.

DiscoveryProbe™ DNA Damage/DNA Repair Library

L1033-.25 250 uL/well(10 mM solution)
EUR 12464
Description: A unique collection of 481 DNA Damage/DNA repair-related compounds for DNA damage/DNA repair research.

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

DNA Polymerase

ABF5235 100 ug
EUR 525.6

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

abx461001-1096tests 10 × 96 tests Ask for price

DNA Polymerase

abx461001-596tests 5 × 96 tests Ask for price

DNA Polymerase

abx461001-96tests 96 tests
EUR 425

DNA-fectamine

DNAF001 1ml
EUR 334.57

DNA-Spermidine

T31554-10mg 10mg Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-1g 1g Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-1mg 1mg Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-50mg 50mg Ask for price
Description: DNA-Spermidine

DNA-Spermidine

T31554-5mg 5mg Ask for price
Description: DNA-Spermidine

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)

TG2000G-7KIT 2000 assays
EUR 3412.8

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)

TG2000G-5KIT 1000 assays
EUR 1934.4

Taq Plus DNA Polymerase (Taq Plus DNA) Protein

abx073522-100tests 100 tests
EUR 225

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-YP889065HU
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-BP889065HU
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus

Human DNA-directed DNA/RNA polymerase mu (POLM)

1-CSB-EP889065HU
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

DNA Amplification Kit with Taq DNA Polymerase, 100app

PL1202-K each Ask for price

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)

TG2000G-1KIT 100 assays
EUR 456

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)

TG2000G-3KIT 500 assays
EUR 1182

DNA nucleotidylexotransferase

AP88338 1mg
EUR 2640

DNA nucleotidylexotransferase

AP88424 1mg
EUR 2640

Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled

AD-155-U Custom Ask for price

Recombinant human DNA-directed DNA/RNA polymerase mu

P2815 100ug Ask for price
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu

Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated

AD-155-B Custom Ask for price

Mouse Anti-DNA Antibody (DNA) ELISA Kit

abx257816-96tests 96 tests
EUR 895.2

pUC 18 DNA

MSD15 50ug
EUR 146.65

pUC 19 DNA

MSD16 50ug
EUR 136.21

pUC 57 DNA

MSD17 50ug
EUR 136.21

DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel

L1007-5 5 mg/well
EUR 7172.4
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc.

Soil DNA Kit

AGDK-0016 Kit Ask for price
Description: Soil DNA Kit offers a rapid and easy-to-use method for the isolation of microbial genomic DNA from all soil samples. This kit is based on spin column chromatography as the separation matrix. After cell lysis, the DNA purification process can be completed in fewer than 20 minutes. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

Soil DNA Kit

AGDK-0035 Kit Ask for price
Description: Soil DNA Kit is designed for isolation of high quality DNA from from soil samples. The kit utilizes our unique high-capacity magnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. After cell lysis, the DNA purification process can be completed in fewer than 20 minutes. The purified DNA is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.

Taq Plus DNA

enz-309 250U
EUR 60
Description: Recombinant Taq Plus DNA Polymerase

DNA Pure Kit

FYG601-100P 100 Preps Ask for price

DNA Pure Kit

FYG601-300P 300 Preps Ask for price

2K DNA Marker

20-abx098045
  • Ask for price
  • Ask for price
  • 2.5 ml
  • 500 ul

5K DNA Marker

20-abx098048
  • Ask for price
  • Ask for price
  • 2.5 ml
  • 500 ul

T4 DNA Ligase

abx098136-10kU 10 kU
EUR 385.2

T4 DNA Ligase

abx098136-20Ku 20 Ku
EUR 477.6

DNA Assay Kit

6023 1 kit
EUR 113
Description: Tissue Extract, Cultured Cells

Phage λ-DNA

301-025 500 µg
EUR 122.4

Phage λ-DNA

301-100 5x500µg
EUR 426

T4 DNA ligase

402-002 400 WEISS UNITS
EUR 75.6

T4 DNA ligase

402-010 2000 WEISS UNITS
EUR 184.8

DNA Ligase IV

E8ET1705-77 100ul
EUR 275
Description: Available in various conjugation types.

DNA Ligase I

E8ET7107-14 100ul
EUR 275
Description: Available in various conjugation types.

DNA ligase 1

AP82345 1mg
EUR 2640

DNA ligase 3

AP82579 1mg
EUR 2640

DNA ligase 3

AP82720 1mg
EUR 2640

DNA ligase 1

AP82852 1mg
EUR 2640

DNA ligase 1

AP83036 1mg
EUR 2640

T4 DNA Ligase

C301-01 40,000 U
EUR 29

Viral DNA Kit

AGDK-0010 Kit Ask for price
Description: Viral DNA Kit offers a rapid and easy-to-use method for the isolation of high quality virus DNA from 1-200ul serum, plasma, and cell-free medium. This kit is based on spin column chromatography as the separation matrix. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

Plant DNA Kit

AGDK-0011 Kit Ask for price
Description: Plant DNA Kit is designed for isolation of high quality DNA from from a wide variety of plant species. Up to 100 mg of wet samples or 30 mg dry samples can be processed in less than 30 minutes.This kit is based on spin column chromatography as the separation matrix. This kit uses a novel and proprietary method to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant sample.The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

Plant DNA Kit

AGDK-0028 Kit Ask for price
Description: Plant DNA Kit is designed for isolation of high quality DNA from from a wide variety of plant species. Up to 100 mg of wet samples or 30 mg dry samples can be processed in less than 30 minutes. The kit utilizes our unique high-capacity magnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA is completely compatible with downstream application such as PCR, qPCR, genotyping, sequencing, Southern Blot analysis, and more.

T4 DNA Ligase

EN-149L 5 x 400Weiss units
EUR 149.7
Description: E. coli lambda lysogen NM 989

T4 DNA Ligase

EN-149S 400Weiss units
EUR 37.6
Description: E. coli lambda lysogen NM 989

T4 DNA Ligase

G467 200 U (200 μl)
EUR 60
Description: T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

T4 DNA Ligase

N103-01 600,000 U
EUR 468

T4 DNA Ligase

RK21501 80000U
EUR 10

DNA Assay Kit

Z5030023 250 assays
EUR 636

T4 DNA ligase

MBT091-10000U 1 unit
EUR 239.21
Description: T4 DNA ligase

T4 DNA ligase

MBT091-1000U 1 unit
EUR 37.28
Description: T4 DNA ligase

T4 DNA Ligase

abx098136-100l 100 µl
EUR 637.5

T4 DNA Ligase

abx098136-1ml 1 ml Ask for price

T4 DNA Ligase

abx098136-200l 200 µl
EUR 675

15K DNA Marker

20-abx098049
  • Ask for price
  • Ask for price
  • 2.5 ml
  • 500 ul

1Kb DNA Marker

20-abx098050
  • Ask for price
  • Ask for price
  • 2.5 ml
  • 500 ul

1Kb DNA Marker

D1101-050 50㎍
EUR 157.2

1Kb DNA Marker

D1101-250 5X50㎍
EUR 360

DNA LADDER (1KB)

IB01351 50uG/500uL
EUR 61.65
Description: [FR]

SYBR DNA Stain

PCR-273 5 x 1,8ml
EUR 48.2

Taq DNA Ligase

RK20502 2000U
EUR 105

Genomic DNA Kit

20-abx098076
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

DNA PK Antibody

abx149863-100ug 100 ug
EUR 526.8

DNA PK antibody

20R-1466 100 ug
EUR 807.6
Description: Rabbit polyclonal DNA PK antibody

DNA PK Antibody

E18-5340-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA PK Antibody

E18-5340-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA PK Antibody

AF5340 200ul
EUR 420

DNA PK Antibody

AF5340-100ul 100ul
EUR 280

DNA PK Antibody

AF5340-200ul 200ul
EUR 350

DNA PK Antibody

ABF5340 100 ug
EUR 525.6

DNA- PK Antibody

ABF0550 100 ug
EUR 525.6

Calf thymus DNA

HY-109517 100mg
EUR 2292

DNA LADDER (100bp)

IB01301 50uG/500uL
EUR 59.65
Description: [FR]

DNA LADDER (100bp)

IB01302 50uG/500uL
EUR 56.1
Description: (NO LOADING DYE) [FR]

GENOMIC DNA KIT

IB47250 2 X 96 PREP KIT
EUR 759.68

GENOMIC DNA KIT

IB47251 4 X 96 PREP KIT
EUR 1464.04

GENOMIC DNA KIT

IB47252 10 X 96 PREP KIT
EUR 3254.61

ELK Genomic DNA

GE-240 0.1mg
EUR 177

DNA Loading Dye

G030 6X; 3 x 1.0 ml
EUR 37
Description: 6X Loading dye is most commonly used in order to monitor the progress of an electrophoresis run. It is combined with DNA samples in order to ensure that samples do not diffuse away when being loaded and also to provide easy visualization during loading.

Consists of:

Glycerol, Tris-HCl, and Bromophenol blue.

Fig Genomic DNA

PLG-1042 0.1mg
EUR 307

Oat Genomic DNA

PLG-1096 0.1mg
EUR 307

Rye Genomic DNA

PLG-1097 0.1mg
EUR 307

Pea Genomic DNA

PLG-1141 0.1mg
EUR 307

Cat Genomic DNA

GC-130 0.1mg
EUR 177

E.coli DNA Ligase

RK20501 1000U
EUR 20.5

DNA, sodium salt

RM511-10G 1 unit
EUR 40.32
Description: DNA, sodium salt

DNA, sodium salt

RM511-25G 1 unit
EUR 87.34
Description: DNA, sodium salt

DNA, sodium salt

RM511-5G 1 unit
EUR 23.74
Description: DNA, sodium salt

DNA, sodium salt

RM362-500MG 1 unit
EUR 352.9
Description: DNA, sodium salt

The dUTPs label with analog zwitterionic electroneutral of Cy3 or Cy5 fluorophores Taq polymerase is used by approximately one order of magnitude more effective than dUTPs analog labeled with a negatively charged of Cy3 or Cy5. Nucleotidyl transferase activity of Taq polymerase were also observed and resulted in the addition dumps electroneutral labeled with fluorophores or positively charged to the 3 ‘end of the DNA.