A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.

Polymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic acids DNA or RNA strand using the base-pairing interactions. One of the polymerase enzyme, Taq DNA polymerase, originally isolated from Thermus aquaticus (Taq) is an enzyme widely used in molecular biology so far.

The properties of thermostable enzymes has contributed majorly to specificity, automation, and efficacy of the polymerase chain reaction (PCR), making it a powerful tool for molecular biology research today throughout the world. Purification of Taq DNA polymerase from the original host results in low yields, labor and time consumption.

Therefore, much research has been done before to get this enzyme using an alternative host. So far, all of the existing methodology is more laborious, time consuming and requires a heavy cost. We use a new approach to purifying the enzyme with relatively high efficiency, yield and minimum time consumption using Escherichia coli (E. coli) as an alternative host.

We cloned 2500 base pairs of DNA Taq polymerase gene into pGEX-4T-1 vector containing GST-tag, downstream of tac promoter and expressed using isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer. The enzyme was purified using chromatography efficient new approach and use in routine PCR tests in our laboratory. Our findings suggest a new approach to facilitate the availability of polymerases for molecular and diagnostic studies. In the future, may be used for purification of other recombinant peptides or proteins used in structural biology and proteomics research-based.

A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli.

Outside Chelation: EDTA binds Closely Taq DNA Polymerase, Mutt and dUTPase and direct Inhibiting Activity dNTPase.

EDTA is commonly used as a metal ion chelator efficient enzyme cofactor. It is highly soluble, optically inactive and do not interfere with most chemicals used in making standard buffer EDTA common options for generating metal-free conditions for biochemical and biophysical investigations.

However, controversy in the literature about the activity of the metal-free enzyme is achieved by using EDTA or otherwise called our attention to the alleged effects of external EDTA chelation. Here, we show that EDTA compete for sites of hydrolases dUTPase nucleotide nucleotide binding by developing a network of interactions within the active site similar to the substrate.

To reach these findings, we apply the kinetics and molecular docking technique uses two different dUTPases. Furthermore, we directly measured the binding of EDTA to dUTPases and two other dNTPases, Taq polymerase and Mutt using isothermal titration calorimetry. EDTA binds proven exothermic and enthalpy driven primarily by submicromolar dissociation constant is much lower than with an enzyme: substrate or Mg: EDTA complex. Control of protein, including ATPase, do not interact with EDTA. Our findings indicate that EDTA can act as a selective inhibitor of the enzyme hydrolysis of dNTPs and urges a rethinking of the use of EDTA in enzymatic trial.

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (FITC)
MBS6335634-02mL 0.2mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (FITC)
MBS6335634-5x02mL 5x0.2mL
EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (HRP)
MBS6335635-02mL 0.2mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (HRP)
MBS6335635-5x02mL 5x0.2mL
EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (Biotin)
MBS6335633-02mL 0.2mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (Biotin)
MBS6335633-5x02mL 5x0.2mL
EUR 4250

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)
MBS6008714-01mg 0.1(mg
EUR 520

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)
MBS6008714-5x01mg 5x0.1mg
EUR 2185

DNA Polymerase
ABF5235 100 ug
EUR 525.6

DNA Polymerase
ABD3082 100 ug
EUR 525.6

DNA Polymerase
abx461001-1096tests 10 × 96 tests Ask for price

DNA Polymerase
abx461001-596tests 5 × 96 tests Ask for price

DNA Polymerase
abx461001-96tests 96 tests
EUR 425

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 405)
MBS6335636-01mL 0.1mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 405)
MBS6335636-5x01mL 5x0.1mL
EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 490)
MBS6335637-01mL 0.1mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 490)
MBS6335637-5x01mL 5x0.1mL
EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 550)
MBS6335638-01mL 0.1mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 550)
MBS6335638-5x01mL 5x0.1mL
EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 650)
MBS6335639-01mL 0.1mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 650)
MBS6335639-5x01mL 5x0.1mL
EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 750)
MBS6335640-01mL 0.1mL
EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 750)
MBS6335640-5x01mL 5x0.1mL
EUR 4250

POLL, CT (DNA Polymerase lambda, Pol Lambda, DNA Polymerase beta-2, Pol beta2, DNA Polymerase kappa)
MBS6009414-005mg 0.05(mg
EUR 745

POLL, CT (DNA Polymerase lambda, Pol Lambda, DNA Polymerase beta-2, Pol beta2, DNA Polymerase kappa)
MBS6009414-5x005mg 5x0.05mg
EUR 3195

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112)
MBS6011074-01mg 0.1(mg
EUR 630

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112)
MBS6011074-5x01mg 5x0.1mg
EUR 2680

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B)
MBS6003978-01mg 0.1(mg
EUR 630

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B)
MBS6003978-5x01mg 5x0.1mg
EUR 2680

DNA polymerase mu
E8ER1907-58 100ul
EUR 275
Description: Available in various conjugation types.

DNA Polymerase I
E1080-01 500U
EUR 54.5
Description: DNA-dependent DNA polymerase with inherent 3'->5' and 5'->3' exonuclease activity (E. coli).

DNA Polymerase I
E1080-02 2500U
EUR 215.82
Description: DNA-dependent DNA polymerase with inherent 3'->5' and 5'->3' exonuclease activity (E. coli).

T4 DNA Polymerase
E1100-01 200U
EUR 64.31
Description: T4 DNA Polymerase exhibits very strong 3'->5' exonuclease activity

T4 DNA Polymerase
E1100-02 1000U
EUR 259.42
Description: T4 DNA Polymerase exhibits very strong 3'->5' exonuclease activity

T4 DNA polymerase
N101-01 2000 U
EUR 223

T4 DNA Polymerase
M1211-100 each
EUR 385.2

DNA Polymerase I
MBS653843-2500Units 2500Units
EUR 865

DNA Polymerase I
MBS653843-500Units 500Units
EUR 425

DNA Polymerase I
MBS653843-5x2500Units 5x2500Units
EUR 3750

DNA Polymerase T4
MBS653894-100Units 100Units
EUR 375

DNA Polymerase T4
MBS653894-5x100Units 5x100Units
EUR 680

DNA polymerase mu
MBS8577451-01mL 0.1mL
EUR 345

DNA polymerase mu
MBS8577451-01mLAF405L 0.1mL(AF405L)
EUR 565

DNA polymerase mu
MBS8577451-01mLAF405S 0.1mL(AF405S)
EUR 565

DNA polymerase mu
MBS8577451-01mLAF610 0.1mL(AF610)
EUR 565

DNA polymerase mu
MBS8577451-01mLAF635 0.1mL(AF635)
EUR 565

SD DNA Polymerase
MBS430264-500Units 500Units
EUR 205

SD DNA Polymerase
MBS430264-5x500Units 5x500Units
EUR 785

Taq DNA Polymerase
9001-2500 each
EUR 614.4

Taq DNA Polymerase
9001-500 each
EUR 229.2

PFU DNA Polymerase
9003-2500 each
EUR 614.4

PFU DNA Polymerase
9003-500 each
EUR 216

Laq? DNA Polymerase
9004-2500 each
EUR 732

Laq? DNA Polymerase
9004-500 each
EUR 261.6

DNA polymerase ? (Rat)
10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)
10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase
BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase
BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase
BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase
BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
E2500-01 200U
EUR 31.61
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase
E2500-02 1000U
EUR 120.99
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase
E2500-03 5000U
EUR 599.5
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase
E2500-04 500U
EUR 69.76
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Tfl DNA Polymerase
EK1112-01 200U
EUR 68.67
Description: Tfl DNA Polymerase Kit containing polymerase and dNTPs

Tfl DNA Polymerase
EK1112-02 1000U
EUR 279.04
Description: Tfl DNA Polymerase Kit containing polymerase and dNTPs

Pfu DNA Polymerase
EK1114-01 100U
EUR 26.16
Description: Pfu DNA Polymerase Kit containing polymerase and dNTPs

Pfu DNA Polymerase
EK1114-02 500U
EUR 104.64
Description: Pfu DNA Polymerase Kit containing polymerase and dNTPs

Pfu DNA Polymerase
EK1114-03 2500U
EUR 491.59
Description: Pfu DNA Polymerase Kit containing polymerase and dNTPs

Tth DNA Polymerase
EK1115-01 100U
EUR 57.77
Description: Tth DNA Polymerase Kit containing polymerase and dNTPs

Tth DNA Polymerase
EK1115-02 500U
EUR 236.53
Description: Tth DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-01 200U
EUR 40.33
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-02 1000U
EUR 163.5
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-03 5000U
EUR 809.87
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-04 500U
EUR 90.47
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EQ005-100ul500U 100μl/500U
EUR 15

Taq DNA Polymerase
EQ005-500ul2500U 500μl/2500U
EUR 50

Pfu DNA Polymerase
EQ008-100ul 100μl
EUR 68

Pfu DNA Polymerase
EQ008-50ul 50μl
EUR 38

Tfl DNA Polymerase
E1112-01 200U
EUR 59.95
Description: Native Thermus flavus DNA polymerase (5.0 U/ul)

Tfl DNA Polymerase
E1112-02 1000U
EUR 237.62
Description: Native Thermus flavus DNA polymerase (5.0 U/ul)

Pfu DNA Polymerase
E1114-01 100U
EUR 23.98
Description: Highly thermostable DNA polymerase for PCR applications requiring high fidelity,proofreading activity; (5.0 U/μl)

Pfu DNA Polymerase
E1114-02 500U
EUR 90.47
Description: Highly thermostable DNA polymerase for PCR applications requiring high fidelity,proofreading activity; (5.0 U/μl)

Pfu DNA Polymerase
E1114-03 2500U
EUR 443.63
Description: Highly thermostable DNA polymerase for PCR applications requiring high fidelity,proofreading activity; (5.0 U/μl)

Tth DNA Polymerase
E1115-01 100U
EUR 54.5
Description: Thermus thermophilus DNA polymerase (5.0 U/μl)

Tth DNA Polymerase
E1115-02 500U
EUR 215.82
Description: Thermus thermophilus DNA polymerase (5.0 U/μl)

Bsu DNA Polymerase
E1450-01 200U
EUR 54.5
Description: Large fragment of mesophilic B. subtilis DNA Polymerase I, which lacks the 5'->3'exonuclease activity and naturally lacks the 3'->5' exonuclease activity

Bsu DNA Polymerase
E1450-02 1000U
EUR 215.82
Description: Large fragment of mesophilic B. subtilis DNA Polymerase I, which lacks the 5'->3'exonuclease activity and naturally lacks the 3'->5' exonuclease activity

Taq DNA Polymerase
G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase
EQ005 100μl/500U
EUR 50

Pfu DNA Polymerase
EQ008 50μl
EUR 68

Pfu DNA Polymerase
enz-265 100U
EUR 145
Description: Recombinant Pfu DNA Polymerase

Taq DNA Polymerase
L7051001 1000 unit
EUR 167

Taq DNA Polymerase
L7051200 200 unit
EUR 43

Pfu DNA Polymerase
S116 1x250 units
EUR 91.2

Pfu DNA Polymerase
S117 2x250 units
EUR 112.8

Pfu DNA Polymerase
S118 10x250 units
EUR 363.6

Bst DNA Polymerase
S600 2000 U
EUR 128.4

Taq DNA Polymerase
abx299725-100g 100 µg Ask for price

Taq DNA Polymerase
abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase
abx299725-50g 50 µg Ask for price

DNA Polymerase Mix
abx461002-1096tests 10 × 96 tests Ask for price

DNA Polymerase Mix
abx461002-596tests 5 × 96 tests Ask for price

DNA Polymerase Mix
abx461002-96tests 96 tests
EUR 1262.5

Taq DNA Polymerase
abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase
abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase
abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase
MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase
MBS157418-1000Units 1000Units
EUR 190

Taq DNA Polymerase
MBS157418-3000Units 3000Units
EUR 245

Taq DNA Polymerase
MBS157418-500Units 500Units
EUR 175

Taq DNA Polymerase
MBS157418-5x3000Units 5x3000Units
EUR 780

Taq DNA Polymerase
MBS402024-500Units 500Units
EUR 170

Taq DNA Polymerase
MBS402024-50Units 50Units
EUR 160

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (AP)
MBS6133044-01mL 0.1(mL
EUR 875

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (AP)
MBS6133044-5x01mL 5x0.1mL
EUR 3800

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (PE)
MBS6159559-01mL 0.1(mL
EUR 875

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (PE)
MBS6159559-5x01mL 5x0.1mL
EUR 3800

DNA Polymerase beta
E8ET1705-12 100ul
EUR 275
Description: Available in various conjugation types.

MyFi DNA Polymerase
BIO-21117 250 Units Ask for price

MyFi DNA Polymerase
BIO-21117/S Sample Ask for price

MyFi DNA Polymerase
BIO-21118 500 Units Ask for price

MyFi DNA Polymerase
BIO-21119 2500 Units Ask for price

DNA Polymerase Beta
E1077-01 50U
EUR 251.79
Description: Human DNA Polymerase

DNA Polymerase Beta
E1077-02 200U
EUR 863.28
Description: Human DNA Polymerase

DNA Polymerase, iota
MBS614888-005mL 0.05mL
EUR 560

DNA Polymerase, iota
MBS614888-5x005mL 5x0.05mL
EUR 2360

DNA Polymerase, beta
MBS632197-01mg 0.1mg
EUR 825

DNA Polymerase, beta
MBS632197-5x01mg 5x0.1mg
EUR 3560

DNA Polymerase, beta
MBS631586-05mL 0.5mL
EUR 745

DNA Polymerase, beta
MBS631586-5x05mL 5x0.5mL
EUR 3205

DNA Polymerase beta
MBS8565555-01mL 0.1mL
EUR 345

DNA Polymerase beta
MBS8565555-01mLAF405L 0.1mL(AF405L)
EUR 565

DNA Polymerase beta
MBS8565555-01mLAF405S 0.1mL(AF405S)
EUR 565

DNA Polymerase beta
MBS8565555-01mLAF610 0.1mL(AF610)
EUR 565

DNA Polymerase beta
MBS8565555-01mLAF635 0.1mL(AF635)
EUR 565

DNA Polymerase, beta
MBS624860-01mL 0.1mL
EUR 625

DNA Polymerase, beta
MBS624860-5x01mL 5x0.1mL
EUR 2665

Taq DNA Polymerase (3000U)
9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)
9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)
9K-001-0035 1000U
EUR 418.62

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) APC
MBS6138343-01mL 0.1(mL
EUR 875

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) APC
MBS6138343-5x01mL 5x0.1mL
EUR 3800

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (AP)
MBS6133040-01mL 0.1(mL
EUR 875

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (AP)
MBS6133040-5x01mL 5x0.1mL
EUR 3800

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (FITC)
MBS6148949-01mL 0.1(mL
EUR 875

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (FITC)
MBS6148949-5x01mL 5x0.1mL
EUR 3800

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (HRP)
MBS6154252-01mL 0.1(mL
EUR 875

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (HRP)
MBS6154252-5x01mL 5x0.1mL
EUR 3800

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (PE)
MBS6159555-01mL 0.1(mL
EUR 875

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B) (PE)
MBS6159555-5x01mL 5x0.1mL
EUR 3800

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) APC
MBS6138347-01mL 0.1(mL
EUR 875

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) APC
MBS6138347-5x01mL 5x0.1mL
EUR 3800

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (HRP)
MBS6154256-01mL 0.1(mL
EUR 875

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (HRP)
MBS6154256-5x01mL 5x0.1mL
EUR 3800

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (FITC)
MBS6148953-01mL 0.1(mL
EUR 875

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112) (FITC)
MBS6148953-5x01mL 5x0.1mL
EUR 3800

DNA polymerase delta
E8ER1907-56 100ul
EUR 275
Description: Available in various conjugation types.

Phi29 DNA Polymerase
E014 1000 U (100 μl)
EUR 145
Description: Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.

MyTaq DNA Polymerase
BIO-21105 500 units Ask for price

MyTaq DNA Polymerase
BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase
BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase
BIO-21107 5000 units Ask for price

onTaq DNA Polymerase
E2713-01 200U
EUR 42.51
Description: The modified "hot start" enzyme which is blocked at moderate temperaturesand allows room temperature reaction setup (2.5 U/ul)

onTaq DNA Polymerase
E2713-02 1000U
EUR 167.86
Description: The modified "hot start" enzyme which is blocked at moderate temperaturesand allows room temperature reaction setup (2.5 U/ul)

onTaq DNA Polymerase
E2713-03 5000U
EUR 827.31
Description: The modified "hot start" enzyme which is blocked at moderate temperaturesand allows room temperature reaction setup (2.5 U/ul)

onTaq DNA Polymerase
E2713-04 500U
EUR 94.83
Description: The modified "hot start" enzyme which is blocked at moderate temperaturesand allows room temperature reaction setup (2.5 U/ul)

tiTaq DNA Polymerase
E2715-01 200U
EUR 37.06
Description: New generation "hot start" Taq DNA Polymerase (2.5 U/ul)

tiTaq DNA Polymerase
E2715-02 1000U
EUR 146.06
Description: New generation "hot start" Taq DNA Polymerase (2.5 U/ul)

tiTaq DNA Polymerase
E2715-03 5000U
EUR 719.4
Description: New generation "hot start" Taq DNA Polymerase (2.5 U/ul)

tiTaq DNA Polymerase
E2715-04 500U
EUR 82.84
Description: New generation "hot start" Taq DNA Polymerase (2.5 U/ul)

phi29 DNA Polymerase
E1401-01 250U
EUR 54.5
Description: A highly processive recombinant polymerase with exceptional strand displacement activity

phi29 DNA Polymerase
E1401-02 1250U
EUR 215.82
Description: A highly processive recombinant polymerase with exceptional strand displacement activity


In order to develop a test to identify the genes responsible for antibiotic resistance in gram-negative bacteria, it has been found that the standard of Taq DNA polymerase (not DNA-free) blaTEM contaminated with fragments of genes that varied in length and number. BlaTEM complete gene sequences are absent or detected in very small amounts. We developed an approach to avoid false positive findings caused by contamination in the polymerase gene sequence blaTEM conventional.