DNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as an important component for the in vitro synthesis of DNA, known as PCR. modern diagnostics, molecular biology and genetic engineering of DNA polymerase needs which demonstrate improved performance.

This study aims to get NeqSSB-TaqS new DNA polymerase fusion of domain Stoffel Taq DNA and single-stranded DNA binding protein from Nanoarchaeum equitans as to significantly improve the properties of the DNA polymerase. Stoffel Taq DNA coding sequence specific DNA polymerase and DNA-binding proteins Nanoarchaeum equitans (NeqSSB-like protein) fused. A new recombinant genes obtained were cloned into pET-30 Ek / LIC vector and introduced into E. coli for expression. Purified recombinant enzymes and enzymatic properties including the activity of DNA polymerase, PCR amplification level, thermostability, processivity and resistance to inhibitors, were tested.

The results of the target protein around 18 mg / l after 24 hours of IPTG induction. The specific activity of the polymerase is 2200 U / mg. Recombinant NeqSSB-TaqS exhibited a higher level extensions (1000 bp template 20 s), processivity (19 nt), thermostability (half-life 35 minutes at 95 ° C) and a higher tolerance for PCR inhibitors (0.3 to 1.25 % of whole blood, 0.84 to 13.5 ug heparin lactoferrin and 4.7 to 150 ng) of Taq DNA polymerase Stoffel.

In addition, our research shows that NeqSSB-TaqS DNA polymerase has a high degree of flexibility in relation to the Mg 2+ ions (from 1 to 5 mM) and KCl or (NH4) 2SO4 salt (more than 60 mM and 40 mM, respectively ). NeqSSB-TaqS using DNA polymerase Taq DNA polymerase can not be a better choice in many applications of PCR.

Data from self-made Taq DNA polymerase prepared for screening purposes.

DNA analysis is the main procedure in genetic engineering. This time the analysis is often done by PCR with Taq DNA polymerase. Although the price of the last enzyme is quite low, the demand for analysis of various results in a lot of spending money that is not affordable for many laboratories. Meanwhile, many screening duties do not require highly purified enzyme.

Taking into account the unique nature of the enzyme that allows for little simplifies the production without the use of expensive techniques such as column chromatography or the length and / or dialysis. The following data routine use of Taq DNA polymerase is prepared in accordance with the protocol developed in our laboratory are presented.

protocols only take a few hours to realize and does not need to be a qualified technician or costly equipment. But give an enzyme preparation suitable for most screening purposes. Taq DNA polymerase isolated stock can be stored as ammonium sulfate suspension in the refrigerator for a long time, not less than 6 months. Working enzyme solution prepared from stock suspension on demand, no more than once a month and can be stored well in the refrigerator.

Long Amp DNA polymerase
MBT069-500U	EWC Diagnostics	1 unit	EUR 88.98
Description: Long Amp DNA polymerase

Long Taq DNA Polymerase
MBS355971-3000Units	MyBiosource	3000Units	EUR 445

Long Taq DNA Polymerase
MBS355971-500Units	MyBiosource	500Units	EUR 235

Long Taq DNA Polymerase
MBS355971-5x3000Units	MyBiosource	5x3000Units	EUR 1700

ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U	ACTGene	each	EUR 272.4

ACTaq™ Long DNA Polymerase, 1000U
E2200	ACTGene	1000U	EUR 160.47

ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U	ACTGene	each	EUR 126

ACTaq™ Taq Blue DNA Polymerase, 1000U
E2100B	ACTGene	1000U	EUR 153.24

Long-Range DNA Polymerase (PCR)
G460	ABM	250 U (50 ul)	EUR 93.6

Long-Range DNA Polymerase (PCR)
G461	ABM	1000 U (200 ul)	EUR 183.6

Long TAQ DNA Polymerase (5U/mcl)
15367	Sisco Laboratories	250 Units	EUR 28.22
Description: Part E

Long TAQ DNA Polymerase (5U/mcl)
15367-1	Sisco Laboratories	1000 Units	EUR 102.63
Description: Part E

Long-Taq DNA Polymerase (5U/uL)
A4186-250U	Biomatik Corporation	250U	EUR 66
Description: Biotechnology

Long Range DNA Polymerase (500 U | 400 reactions)
BRBR0300302	Westburg	each	EUR 388.59

O' in 1 DNA Polymerase Premix 2xwi Blue Dye
FYT206-100P	Yeastern Biotech	100 Preps 1.25ml x 2 vials	Ask for price

Long Range DNA Polymerase (100 U | 80 reactions)
BRBR0300301	Westburg	each	EUR 95.38

Long TAQ DNA Polymerase (5U/mcl) (w/dNTP)
94126	Sisco Laboratories	250 Units	EUR 42.76
Description: Part E

Long TAQ DNA Polymerase (5U/mcl) (w/dNTP)
94126-1	Sisco Laboratories	1000 Units	EUR 128.29
Description: Part E

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S111	GeneOn	500 units	EUR 63.6

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S112	GeneOn	5x500 units	EUR 148.8

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S128	GeneOn	20x500 units	EUR 487.2

Maximo Blue-Taq DNA Polymerase (ready-to-load, conc. 1u/µl)
S129	GeneOn	100x500 units or as bulk	EUR 2142

LongerAmp Taq DNA Polymerase
RK20680	Abclonal	1000U	EUR 130.8

amfiSure LQ Long PCR Polymerase
P0326-010	GenDepot	100 units	EUR 230.4

amfiSure LQ Long PCR Polymerase
P0326-025	GenDepot	250 units	EUR 400.8

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa)
MBS648582-02mL	MyBiosource	0.2mL	EUR 695

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa)
MBS648582-5x02mL	MyBiosource	5x0.2mL	EUR 2975

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (AP)
MBS6335631-02mL	MyBiosource	0.2mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (AP)
MBS6335631-5x02mL	MyBiosource	5x0.2mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (PE)
MBS6335641-02mL	MyBiosource	0.2mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (PE)
MBS6335641-5x02mL	MyBiosource	5x0.2mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (APC)
MBS6335632-02mL	MyBiosource	0.2mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (APC)
MBS6335632-5x02mL	MyBiosource	5x0.2mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (FITC)
MBS6335634-02mL	MyBiosource	0.2mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (FITC)
MBS6335634-5x02mL	MyBiosource	5x0.2mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (HRP)
MBS6335635-02mL	MyBiosource	0.2mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (HRP)
MBS6335635-5x02mL	MyBiosource	5x0.2mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (Biotin)
MBS6335633-02mL	MyBiosource	0.2mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (Biotin)
MBS6335633-5x02mL	MyBiosource	5x0.2mL	EUR 4250

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)
MBS6008714-01mg	MyBiosource	0.1(mg	EUR 520

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH)
MBS6008714-5x01mg	MyBiosource	5x0.1mg	EUR 2185

DNA Polymerase
ABF5235	Lifescience Market	100 ug	EUR 525.6

DNA Polymerase
ABD3082	Lifescience Market	100 ug	EUR 525.6

DNA Polymerase
abx461001-1096tests	Abbexa	10 × 96 tests	Ask for price

DNA Polymerase
abx461001-596tests	Abbexa	5 × 96 tests	Ask for price

DNA Polymerase
abx461001-96tests	Abbexa	96 tests	EUR 425

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 405)
MBS6335636-01mL	MyBiosource	0.1mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 405)
MBS6335636-5x01mL	MyBiosource	5x0.1mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 490)
MBS6335637-01mL	MyBiosource	0.1mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 490)
MBS6335637-5x01mL	MyBiosource	5x0.1mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 550)
MBS6335638-01mL	MyBiosource	0.1mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 550)
MBS6335638-5x01mL	MyBiosource	5x0.1mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 650)
MBS6335639-01mL	MyBiosource	0.1mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 650)
MBS6335639-5x01mL	MyBiosource	5x0.1mL	EUR 4250

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 750)
MBS6335640-01mL	MyBiosource	0.1mL	EUR 980

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (MaxLight 750)
MBS6335640-5x01mL	MyBiosource	5x0.1mL	EUR 4250

POLL, CT (DNA Polymerase lambda, Pol Lambda, DNA Polymerase beta-2, Pol beta2, DNA Polymerase kappa)
MBS6009414-005mg	MyBiosource	0.05(mg	EUR 745

POLL, CT (DNA Polymerase lambda, Pol Lambda, DNA Polymerase beta-2, Pol beta2, DNA Polymerase kappa)
MBS6009414-5x005mg	MyBiosource	5x0.05mg	EUR 3195

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112)
MBS6011074-01mg	MyBiosource	0.1(mg	EUR 630

POLQ (DNA Polymerase theta, DNA Polymerase eta, POLH, DKFZp781A0112)
MBS6011074-5x01mg	MyBiosource	5x0.1mg	EUR 2680

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B)
MBS6003978-01mg	MyBiosource	0.1(mg	EUR 630

POLE2 (DPE2, DNA Polymerase epsilon Subunit 2, DNA Polymerase II Subunit 2, DNA Polymerase epsilon Subunit B)
MBS6003978-5x01mg	MyBiosource	5x0.1mg	EUR 2680

DNA polymerase mu
E8ER1907-58	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

DNA Polymerase I
E1080-01	EURx	500U	EUR 54.5
Description: DNA-dependent DNA polymerase with inherent 3'->5' and 5'->3' exonuclease activity (E. coli).

DNA Polymerase I
E1080-02	EURx	2500U	EUR 215.82
Description: DNA-dependent DNA polymerase with inherent 3'->5' and 5'->3' exonuclease activity (E. coli).

T4 DNA Polymerase
E1100-01	EURx	200U	EUR 64.31
Description: T4 DNA Polymerase exhibits very strong 3'->5' exonuclease activity

T4 DNA Polymerase
E1100-02	EURx	1000U	EUR 259.42
Description: T4 DNA Polymerase exhibits very strong 3'->5' exonuclease activity

T4 DNA polymerase
N101-01	Vazyme	2000 U	EUR 223

T4 DNA Polymerase
M1211-100	Biovision	each	EUR 385.2

DNA Polymerase I
MBS653843-2500Units	MyBiosource	2500Units	EUR 865

DNA Polymerase I
MBS653843-500Units	MyBiosource	500Units	EUR 425

DNA Polymerase I
MBS653843-5x2500Units	MyBiosource	5x2500Units	EUR 3750

DNA Polymerase T4
MBS653894-100Units	MyBiosource	100Units	EUR 375

DNA Polymerase T4
MBS653894-5x100Units	MyBiosource	5x100Units	EUR 680

DNA polymerase mu
MBS8577451-01mL	MyBiosource	0.1mL	EUR 345

DNA polymerase mu
MBS8577451-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

DNA polymerase mu
MBS8577451-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

DNA polymerase mu
MBS8577451-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

DNA polymerase mu
MBS8577451-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

SD DNA Polymerase
MBS430264-500Units	MyBiosource	500Units	EUR 205

SD DNA Polymerase
MBS430264-5x500Units	MyBiosource	5x500Units	EUR 785

Taq DNA Polymerase
9001-2500	Biovision	each	EUR 614.4

Taq DNA Polymerase
9001-500	Biovision	each	EUR 229.2

PFU DNA Polymerase
9003-2500	Biovision	each	EUR 614.4

PFU DNA Polymerase
9003-500	Biovision	each	EUR 216

Laq? DNA Polymerase
9004-2500	Biovision	each	EUR 732

Laq? DNA Polymerase
9004-500	Biovision	each	EUR 261.6

DNA polymerase ? (Rat)
10-101	Sceti	20ug	EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)
10-102	Sceti	100ug	EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase
BA00103	Cusabio	500U	EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00104	Bioatlas	1000U	EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00105	Bioatlas	2500U	EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase
BA00503	Bioatlas	500U	EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase
BA00504	Bioatlas	1000U	EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase
BA00505	Bioatlas	2500U	EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10101	Bioatlas	500U	EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10102	Bioatlas	1000U	EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10103	Bioatlas	2000U	EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
E2500-01	EURx	200U	EUR 31.61
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase
E2500-02	EURx	1000U	EUR 120.99
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase
E2500-03	EURx	5000U	EUR 599.5
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase
E2500-04	EURx	500U	EUR 69.76
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Tfl DNA Polymerase
EK1112-01	EURx	200U	EUR 68.67
Description: Tfl DNA Polymerase Kit containing polymerase and dNTPs

Tfl DNA Polymerase
EK1112-02	EURx	1000U	EUR 279.04
Description: Tfl DNA Polymerase Kit containing polymerase and dNTPs

Pfu DNA Polymerase
EK1114-01	EURx	100U	EUR 26.16
Description: Pfu DNA Polymerase Kit containing polymerase and dNTPs

Pfu DNA Polymerase
EK1114-02	EURx	500U	EUR 104.64
Description: Pfu DNA Polymerase Kit containing polymerase and dNTPs

Pfu DNA Polymerase
EK1114-03	EURx	2500U	EUR 491.59
Description: Pfu DNA Polymerase Kit containing polymerase and dNTPs

Tth DNA Polymerase
EK1115-01	EURx	100U	EUR 57.77
Description: Tth DNA Polymerase Kit containing polymerase and dNTPs

Tth DNA Polymerase
EK1115-02	EURx	500U	EUR 236.53
Description: Tth DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-01	EURx	200U	EUR 40.33
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-02	EURx	1000U	EUR 163.5
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-03	EURx	5000U	EUR 809.87
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EK2500-04	EURx	500U	EUR 90.47
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase
EQ005-100ul500U	ELK Biotech	100μl/500U	EUR 15

Taq DNA Polymerase
EQ005-500ul2500U	ELK Biotech	500μl/2500U	EUR 50

Pfu DNA Polymerase
EQ008-100ul	ELK Biotech	100μl	EUR 68

Pfu DNA Polymerase
EQ008-50ul	ELK Biotech	50μl	EUR 38

Tfl DNA Polymerase
E1112-01	EURx	200U	EUR 59.95
Description: Native Thermus flavus DNA polymerase (5.0 U/ul)

Tfl DNA Polymerase
E1112-02	EURx	1000U	EUR 237.62
Description: Native Thermus flavus DNA polymerase (5.0 U/ul)

Pfu DNA Polymerase
E1114-01	EURx	100U	EUR 23.98
Description: Highly thermostable DNA polymerase for PCR applications requiring high fidelity,proofreading activity; (5.0 U/μl)

Pfu DNA Polymerase
E1114-02	EURx	500U	EUR 90.47
Description: Highly thermostable DNA polymerase for PCR applications requiring high fidelity,proofreading activity; (5.0 U/μl)

Pfu DNA Polymerase
E1114-03	EURx	2500U	EUR 443.63
Description: Highly thermostable DNA polymerase for PCR applications requiring high fidelity,proofreading activity; (5.0 U/μl)

Tth DNA Polymerase
E1115-01	EURx	100U	EUR 54.5
Description: Thermus thermophilus DNA polymerase (5.0 U/μl)

Tth DNA Polymerase
E1115-02	EURx	500U	EUR 215.82
Description: Thermus thermophilus DNA polymerase (5.0 U/μl)

Bsu DNA Polymerase
E1450-01	EURx	200U	EUR 54.5
Description: Large fragment of mesophilic B. subtilis DNA Polymerase I, which lacks the 5'->3'exonuclease activity and naturally lacks the 3'->5' exonuclease activity

Bsu DNA Polymerase
E1450-02	EURx	1000U	EUR 215.82
Description: Large fragment of mesophilic B. subtilis DNA Polymerase I, which lacks the 5'->3'exonuclease activity and naturally lacks the 3'->5' exonuclease activity

Taq DNA Polymerase
G009	ABM	1000 U (200 ul)	EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase
EQ005	ELK Biotech	100μl/500U	EUR 50

Pfu DNA Polymerase
EQ008	ELK Biotech	50μl	EUR 68

Pfu DNA Polymerase
enz-265	ProSpec Tany	100U	EUR 145
Description: Recombinant Pfu DNA Polymerase

Taq DNA Polymerase
L7051001	Biochain	1000 unit	EUR 167

Taq DNA Polymerase
L7051200	Biochain	200 unit	EUR 43

Pfu DNA Polymerase
S116	GeneOn	1x250 units	EUR 91.2

Pfu DNA Polymerase
S117	GeneOn	2x250 units	EUR 112.8

Pfu DNA Polymerase
S118	GeneOn	10x250 units	EUR 363.6

Bst DNA Polymerase
S600	GeneOn	2000 U	EUR 128.4

Taq DNA Polymerase
abx299725-100g	Abbexa	100 µg	Ask for price

Taq DNA Polymerase
abx299725-20g	Abbexa	20 µg	EUR 337.5

Taq DNA Polymerase
abx299725-50g	Abbexa	50 µg	Ask for price

DNA Polymerase Mix
abx461002-1096tests	Abbexa	10 × 96 tests	Ask for price

DNA Polymerase Mix
abx461002-596tests	Abbexa	5 × 96 tests	Ask for price

DNA Polymerase Mix
abx461002-96tests	Abbexa	96 tests	EUR 1262.5

Taq DNA Polymerase
abx461029-1096tests	Abbexa	10 × 96 tests	EUR 1787.5

Taq DNA Polymerase
abx461029-596tests	Abbexa	5 × 96 tests	EUR 1062.5

Taq DNA Polymerase
abx461029-96tests	Abbexa	96 tests	EUR 362.5

Taq DNA Polymerase
MB101-0500	Bio-Helix	500 U/100 μl	EUR 10

Taq DNA Polymerase
MBS157418-1000Units	MyBiosource	1000Units	EUR 190

Taq DNA Polymerase
MBS157418-3000Units	MyBiosource	3000Units	EUR 245

Port plant homolog of various animal genes involved in the metabolism of phosphorus, telomere biology and other cellular processes. Compared to experiment with many other multicellular organisms, research in the model plant Arabidopsis thaliana take advantage of a short generation time and increasing warehouse of genetic tools and GMOs, including a large collection of T-DNA knockout and line activation. Available to the public availability of thousands of transgenic Arabidopsis lines provide a unique opportunity to address a number of important biological questions.