Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

BACKGROUND: Detection of bacteria by PCR applied for screening blood and blood products with special attention platelet concentrates. For practical use is expected that the detection system including Gram-positive, Gram-negative and Gram-bacterial non-stainable. It is quite challenging to achieve high sensitivity with clear negative controls with PCR reagents, due mainly Taq DNA polymerase is contaminated with traces of bacteria.

METHODS: Bacterial decontamination of Taq DNA polymerase tested by two different methods using restriction enzymes Sau 3A1 and microfiltration. Besides the commercially available Taq polymerase depleted bacterial DNA was included. A published real-time PCR specific for Gram-negative bacteria adapted to Gram-positive bacteria, including Staphylococcus species specific and Mycobacteria, and is used to fill three Taq polymer-ases discharged from bacteria

RESULTS DNA contamination: Despite published reports of successful DNA decontamination , all three approaches perform poorly in experiments conducted in this study. Sensitivity ranges from about 50-100 colony units (CFU) per PCR reaction to Escherichia coli and Staphylococcus epidermidis, corresponding to 1.250 to 2.500 CFU / ml sample material.

Conclusion: It seems unsatisfactory to receive a high detection limit for the PCR bacteria even if highly multiplexed diagnostics. a reliable method for the decontamination of Taq DNA polymerase is required and will present one important step towards the detection of bacterial DNA with high sensitivity.

Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.
Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

Reduce the reaction volume and increase the concentration of Taq DNA polymerase increases STR DNA profile results from the low source Template real world: telogen hair.

OBJECTIVE
The main method for the analysis of low template DNA (LTDNA) is known as a method of low copy number (LCN), which involves increasing the number of PCR cycles (typically 34). In common with other LTDNA method, characterized by an imbalance LCN profile allele, decline, and the drop outs which require interpretation of complex rules. They often require replicate PCR reactions to produce a “consensus” profile in special facilities. The ideal method for the analysis of LTDNA should raise the profile of the results without a high error rate and is accomplished using the standard amenities, in addition to the minimum cost.


METHOD
In this study, we present a comparison of four methods of variation for amplification of STR from LTDNA broadly used, the kit is available commercially (AmpFℓSTR (®) Profiler Plus (®)): the standard method, the standard method to post a PCR clean up, the method of the LCN, and the reaction volume decreases with increased concentrations of Taq DNA polymerase.


RESULTS
Using the telogen hair-LTDNA common source and reference DNA match, LCN method produces the highest number of appropriate and non-appropriate alleles (ie, down-in). For comparison, the reaction volume was reduced by an increase in Taq Polymerase produce fuller profile and corresponding DNA (all alleles combined) and less off-ladder alleles of a variety of input DNA. In addition, this method results in less non-concordant allele of the LCN and not more than for standard PCR, which indicates that it may be preferable to increase PCR cycles for analysis LTDNA, either with or without consensus profiles and statistical modeling.

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2, Bulk Pack

P0701-151 15,000 units
EUR 1560

amfiEco PCR Premix

P0702-096 96 rxns
EUR 69

amfiEco PCR Premix

P0702-480 5x96 rxns
EUR 300

amfiEco PCR Premix

P0702-960 10x96 rxns
EUR 580

amfiEco PCR Master Mix,8-Strip RTU(2X), for 50ul reaction

P0704-096 96 rxns
EUR 174

amfiXpand Taq DNA Ploymerase Buffer*(2X)

P0332-001 1ml
EUR 121.2

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 78

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 140

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 36

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 59

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 90

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 430

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 745

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-025 250 unit
EUR 99

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-050 2X250 unit
EUR 152

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-100 4X250 Unit
EUR 309

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-250 10X250 Unit
EUR 572

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 143

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-502 2x500 units
EUR 259

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-510 10x500 units
EUR 1089

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-525 25x500 units
EUR 2130

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-550 50x500 units
EUR 4114

Active Taq Polymerase (Taq)

APX161Ge61 10ug
EUR 280

Eukaryotic Taq Polymerase (Taq)

EPX161Ge61 10ug
EUR 200

Eukaryotic Taq Polymerase (Taq)

MBS2125395-001mg 0.01mg
EUR 185

Eukaryotic Taq Polymerase (Taq)

MBS2125395-005mg 0.05mg
EUR 340

Eukaryotic Taq Polymerase (Taq)

MBS2125395-01mg 0.1mg
EUR 515

Eukaryotic Taq Polymerase (Taq)

MBS2125395-02mg 0.2mg
EUR 630

Eukaryotic Taq Polymerase (Taq)

MBS2125395-05mg 0.5mg
EUR 1205

Eukaryotic Taq Polymerase (Taq)

RPU59346-100ug 100ug
EUR 672

Eukaryotic Taq Polymerase (Taq)

RPU59346-1mg 1mg
EUR 2925

Eukaryotic Taq Polymerase (Taq)

RPU59346-50ug 50ug
EUR 550

Recombinant Taq Polymerase (Taq)

RPX161Ge01 10ug
EUR 160

Taq

E4A04A03 50ug
EUR 255
Description: Available in various conjugation types.

pTrc- Taq

PVT10500 2 ug
EUR 486

Taq Plasmid

PVT7001 2 ug
EUR 614.4

Taq Polymerase

MBT060-1000U 1 unit
EUR 52.73
Description: Taq Polymerase

Taq Polymerase

MBT060-5000U 1 unit
EUR 204.76
Description: Taq Polymerase

Taq Polymerase

MBT060-500U 1 unit
EUR 35.36
Description: Taq Polymerase

Taq Polymerase

PCR-211L 5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-211S 200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-420-100KU 100 kiLo units
EUR 3944.43

Taq Polymerase

PCR-420-10KU 10kilo units
EUR 788.89

Taq Polymerase

PCR-420-1KU 1kilo unit
EUR 168.06

Taq Polymerase

PCR-420-1MU 1 mio units Ask for price

Taq Plus DNA Polymerase (Taq Plus DNA) Protein

abx073522-100tests 100 tests
EUR 225

Isocitrate Dehydrogenase (Taq) (Thermostable) (rICDH-Taq)

MBS682155-150Units 150Units
EUR 365

Taq HS Mix

abx461040-1096tests 10 × 96 tests Ask for price

Taq HS Mix

abx461040-596tests 5 × 96 tests
EUR 1725

Taq HS Mix

abx461040-96tests 96 tests
EUR 475

Taq Plus DNA

enz-309 250U
EUR 60
Description: Recombinant Taq Plus DNA Polymerase

Taq Core Kit

PCR-214L 5 x 200units
EUR 271.9

Taq Core Kit

PCR-214S 200units
EUR 68.2

Green Taq Mix

P131-01 5 ml
EUR 142.8

Green Taq Mix

P131-02 15 ml
EUR 180

Green Taq Mix

P131-03 50 ml
EUR 297.6

Taq DNA Ligase

3219 1/EA
EUR 180
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. Taq DNA Ligase is active at elevated temperatures (45°C-70°C) Product IncludesTaq DNA Ligase10X Taq DNA Ligase Buffer with NAD+

10x Taq Buffer

BA00151 1.8ml
EUR 66
Description: High purity buffer for various PCR applications.

Taq DNA Ligase

MBS9141677-2000Units 2000Units
EUR 160

Taq DNA Ligase

RK20502 2000U
EUR 106.28

BioReady LA Taq

TRI-A13M2 500U
EUR 85.68

BioReady LA Taq

TRI-A13S2 250U
EUR 45.36

BioReady Taq Mix

TRI-A31M1 200T
EUR 221.76

BioReady Taq Mix

TRI-A31S1 50T
EUR 65.52

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

3243 1000units
EUR 92
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Taq DNA Polymerase

3243d 1000units
EUR 120
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Taq DNA Polymerase

3245 1/EA
EUR 359.2
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Taq DNA Polymerase

3245d 1/EA
EUR 476
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer10mM dNTP Mix

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase

E2500-01 200U
EUR 31.61
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-02 1000U
EUR 120.99
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-03 5000U
EUR 599.5
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-04 500U
EUR 69.76
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

EK2500-01 200U
EUR 40.33
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-02 1000U
EUR 163.5
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-03 5000U
EUR 809.87
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-04 500U
EUR 90.47
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Taq DNA Polymerase

EQ005-100ul500U 100μl/500U
EUR 15

Taq DNA Polymerase

EQ005-500ul2500U 500μl/2500U
EUR 50

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

MBS157418-1000Units 1000Units
EUR 190

Taq DNA Polymerase

MBS157418-3000Units 3000Units
EUR 245

Taq DNA Polymerase

MBS157418-500Units 500Units
EUR 175

Taq DNA Polymerase

MBS157418-5x3000Units 5x3000Units
EUR 780

Taq DNA Polymerase

MBS402024-500Units 500Units
EUR 170

Taq DNA Polymerase

MBS402024-50Units 50Units
EUR 160

Taq Enzyme Antibody

MBS186242-INQUIRE INQUIRE Ask for price

Taq Enzyme Antibody

MBS186243-1mg 1mg
EUR 250

Taq Enzyme Antibody

MBS186243-2x1mg 2x1mg
EUR 370

Taq Enzyme Antibody

MBS186243-5x1mg 5x1mg
EUR 820

Taq-DNA-Polymerase

L7051001 1000 unit
EUR 116.9

Taq-DNA-Polymerase

L7051200 200 unit
EUR 30.1

Champagne Taq antibody

P121-01 500 U
EUR 72.5

Apex Hot Start Taq, 1000u

42-107 2 x 500u/Unit
EUR 232.71
Description: Buffers I & II (Mg Free)

Apex Hot Start Taq, 2500u

42-196 5 x 500u/Unit
EUR 572.98
Description: Buffers I & II (Mg Free)

Apex Hot Start Taq, 5000u

42-210 10 x 500u/Unit
EUR 1075.59
Description: Buffers I & II (Mg Free)

FSP Taq Mix (2x)

11307 1 ml
EUR 41.82
Description: Part E

FSP Taq Mix (2x)

11307-1 5 x1 ml
EUR 187.22
Description: Part E

Taq HS Red Mix

abx461042-1096tests 10 × 96 tests Ask for price

Taq HS Red Mix

abx461042-596tests 5 × 96 tests
EUR 1725

Taq HS Red Mix

abx461042-96tests 96 tests
EUR 475

Taq I unit: 410

YRTAQ1 1 vial Ask for price

Long Taq Mix (2x)

36250 1 ml
EUR 62.69
Description: Part E

Long Taq Mix (2x)

36250-1 5 x1 ml
EUR 154.97
Description: Part E

NXT Taq PCR Kit

E2530-01 100reactions
EUR 47.96
Description: Ready-to-use Solution containing "hot start" NXT Taq DNA Polymerase, reaction buffer,MgCl2 and dNTPs. NXT Taq PCR Kit is designed for fast-cycling PCR on any thermal cycler

NXT Taq PCR Kit

E2530-02 200reactions
EUR 93.74
Description: Ready-to-use Solution containing "hot start" NXT Taq DNA Polymerase, reaction buffer,MgCl2 and dNTPs. NXT Taq PCR Kit is designed for fast-cycling PCR on any thermal cycler

NXT Taq PCR Kit

E2530-03 500reactions
EUR 191.84
Description: Ready-to-use Solution containing "hot start" NXT Taq DNA Polymerase, reaction buffer,MgCl2 and dNTPs. NXT Taq PCR Kit is designed for fast-cycling PCR on any thermal cycler

Taq Blood PCR Kit

abx461037-1096tests 10 × 96 tests Ask for price

Taq Blood PCR Kit

abx461037-596tests 5 × 96 tests Ask for price

Taq Blood PCR Kit

abx461037-96tests 96 tests
EUR 612.5

Taq 2X Master Mix

MBS308023-1000Reactions 1000Reactions
EUR 340

Taq 2X Master Mix

MBS308023-100Reactions 100Reactions
EUR 165

Taq 2X Master Mix

MBS308023-250Reactions 250Reactions
EUR 185

Taq 2X Master Mix

MBS308023-500Reactions 500Reactions
EUR 240

2 × Taq Master Mix

P111-01 5 ml
EUR 36.91

2 × Taq Master Mix

P111-01-51ml 5 × 1 ml
EUR 38.22

2 × Taq Master Mix

P111-02 15 ml
EUR 98.42

2 × Taq Master Mix

P111-03 50 ml
EUR 290

2 × Taq Master Mix

P111-03-501ml 50 × 1 ml
EUR 300.3

Apex Hot Start Taq, 500u

42-106 1 x 500u/Unit
EUR 131.2
Description: Buffers I & II (Mg Free)

Apex Hot Start Taq, 10,000u

42-211 20 x 500u/Unit
EUR 2162.67
Description: Buffers I & II (Mg Free)

Apex Hot Start Taq, 50,000u

42-212 100 x 500u/Unit
EUR 8349.64
Description: Buffers I & II (Mg Free)

Apex Taq, 500Units, 5u/ul

42-800B1 1 x 500u/Unit
EUR 65.71
Description: 10X Standard & NH4 Buffers

Apex Taq, 500Units, 5u/ul

42-800B2 1 x 500u/Unit
EUR 64.49
Description: 10X Standard MgFree&NH4 MgFree

Apex Taq, 500Units, 5u/ul

42-800B3 1 x 500u/Unit
EUR 82.81
Description: 10X Combination Buffer

Apex Taq, 500Units, 5u/ul

42-800B4 1 x 500u/Unit
EUR 63.05
Description: Glycerol Free; No Rxn Buffer

Apex Taq, 1,000Units, 5u/ul

42-801B1 2 x 500u/Unit
EUR 126.43
Description: 10X Standard & NH4 Buffers

Apex Taq, 1,000Units, 5u/ul

42-801B2 2 x 500u/Unit
EUR 129.98
Description: 10X Standard MgFree&NH4 MgFree

Apex Taq, 1,000Units, 5u/ul

42-801B3 2 x 500u/Unit
EUR 132.2
Description: 10X Combination Buffer

Apex Taq, 1,000Units, 5u/ul

42-801B4 2 x 500u/Unit
EUR 134.14
Description: Glycerol Free; No Rxn Buffer

Apex Taq, 5,000Units, 5u/ul

42-802B1 10 x 500u/Unit
EUR 644.58
Description: 10X Standard & NH4 Buffers

Apex Taq, 5,000Units, 5u/ul

42-802B2 10 x 500u/Unit
EUR 555.22
Description: 10X Standard MgFree&NH4 MgFree

Apex Taq, 5,000Units, 5u/ul

42-802B3 10 x 500u/Unit
EUR 549.95
Description: 10X Combination Buffer

Apex Taq, 5,000Units, 5u/ul

42-802B4 10 x 500u/Unit
EUR 536.63
Description: Glycerol Free; No Rxn Buffer

Apex Taq, 10,000Units, 5u/ul

42-803B1 20 x 500u/Unit
EUR 1219.22
Description: 10X Standard & NH4 Buffers

Apex Taq, 10,000Units, 5u/ul

42-803B2 20 x 500u/Unit
EUR 1253.36
Description: 10X Standard MgFree&NH4 MgFree

Apex Taq, 10,000Units, 5u/ul

42-803B3 20 x 500u/Unit
EUR 1428.96
Description: 10X Combination Buffer

Apex Taq, 10,000Units, 5u/ul

42-803B4 20 x 500u/Unit
EUR 1182.48
Description: Glycerol Free; No Rxn Buffer

2×Taq PCR Mixture

E28IT0048 5×1ml
EUR 50.79

Ruby Taq Master (2x)

PCR-164L 20 x 1.25ml
EUR 352.83
Description: Ready-to-Use Mixes for PCR

Ruby Taq Master (2x)

PCR-164S 4 x 1,25ml
EUR 88.15
Description: Ready-to-Use Mixes for PCR

Ruby Taq Master (2x)

PCR-164XL 100ml
EUR 1128.63
Description: Ready-to-Use Mixes for PCR

Wise Taq Premix (96Rx)

9K-002-0028 96Rx, 96prep
EUR 283.94

Wise Taq Premix (576Rx)

9K-002-0032 576Rx, 576prep
EUR 1066.94

Taq 2X PCR MasterMix

G888 400 rxn (10.0 ml)
EUR 60
Description: Taq 2X PCR MasterMix is a ready-to-use MasterMix containing Taq DNA Polymerase (Cat. No. G009) in a uniquely-formulated buffer with gel loading dye. Taq has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

2X PCR Taq MasterMix

G900 25.0 ml (1000 Rxns)
EUR 246

2X PCR Taq MasterMix

G013 5.0 ml (200 Rxns)
EUR 104.4

Taq Polymerase (5U/µl)

MBT060A-1000U 1 unit
EUR 62.03
Description: Taq Polymerase (5U/µl)

Taq Polymerase (5U/µl)

MBT060A-5000U 1 unit
EUR 241.01
Description: Taq Polymerase (5U/µl)

Taq Polymerase (5U/µl)

MBT060A-500U 1 unit
EUR 41.58
Description: Taq Polymerase (5U/µl)

Taq Polymerase(3U/µl)

MBT060B-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060B-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060B-500U 1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)

Taq Polymerase(3U/µl)

MBT060C-500U 1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)

Taq Polymerase(1U/µl)

MBT060D-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060D-5000U 1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060D-500U 1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)

Taq Polymerase(1U/µl)

MBT060E-1000U 1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)


CONCLUSION

Overall, this study highlights the importance and benefits of optimizing PCR conditions and to develop improved laboratory method to amplify and analyze LTDNA.