CONTEXT We have recently shown that the tet family enzymes convert 5-™ 5-hydroxymethylcytosin (5-HMC) to DNA. 5-HMC is present at high levels in embryonic stem
Read MoreWe have developed a general and simple method for directing specific sequence modifications in a plasmid using the amplification approved by the reaction of the
Read MoreViral hemorrhagic fevers (VHFS) are acute infections with high mortality rates. Significant VHF agents are Ebola and Marburg viruses (MBGV / EBOV), Lassa Virus (LASV),
Read MoreA polymerase thermostable DNA which has an associated exonuclease activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus Furiosus (PFU). To test its loyalty, we
Read MoreA set of universal oligonucleotide primers for the retained regions of the eubactterial gene 16S arrnes has been designed for use with the 7700 Real-Time
Read Morechemical modifications to DNA, such as modification 2 ‘, is expected to increase the utility of DNA biotechnology; However, other forms of modification of DNA
Read MoreBACKGROUND: Detection of bacteria by PCR applied for screening blood and blood products with special attention platelet concentrates. For practical use is expected that the
Read MoreDNA polymerases are present in all organisms and enzymes important that synthesize DNA molecules. They are used in various fields of science, especially as an
Read MoreMerging AuNPs to polymerase chain reaction (PCR) has become a promising strategy to develop a sensitive sensing platform, since the desired optical properties of AuNPs
Read MorePolymerases are enzymes that synthesize long chains or polymers of nucleic acids, including DNA or RNA of nucleotides. They assemble to copy the template nucleic
Read More