Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition.

chemical modifications to DNA, such as modification 2 ‘, is expected to increase the utility of DNA biotechnology; However, other forms of modification of DNA is limited by their inability to effectively synthesized by a DNA polymerase enzyme. Previous efforts have identified a mutant Thermus aquaticus DNA polymerase I (Taq) enzyme that can recognize DNA 2’-modified nucleotides.

While these modified nucleotides recognize the mutant enzyme, they are unable to synthesize the full length of the modified DNA; thus, further engineering is required for this enzyme. Here, we describe a comparative biochemical studies that identify the useful properties, but previously uncharacterized, this enzyme; an enzyme, SFM19, able to recognize various 2′-modified nucleotides that is broader than that previously examined, including fluoro, Azido and amino modification.

To understand the molecular origin of these differences, we also identified certain amino acids and combinations of amino acids that contribute most to the unusual activity that were previously grown. Our data indicate that the negatively charged amino acid residues 614 and mutation of steric gate, E615, to Glycine create the optimal combination for the synthesis of modified oligonucleotides. The study provides a better understanding of the origins of the mutation of 2′-modified substrate recognition and identify SFM19 as the best candidate for advanced engineering, whether through rational design or directed evolution.

Taq DNA Polymerase Mutants and 2'-Modified Sugar Recognition.
Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition.

A new iridoid, verbascoside and derivatives with inhibitory activity against Taq DNA polymerase.

DNA polymerases are enzymes that play an important role in DNA metabolism such as replication, repair, transcription, recombination, and chromosome segregation during mitosis. Here we report the isolation of a new iridoid (6-epi-catalpol, 2) and the per-O-acetyl-verbascoside (11) of the aerial parts of Buddleja cordobensis Grisebach (Buddlejaceae). Of the compound 2, we have obtained eight compounds with chemical transformations.

This group of compounds at a concentration of 500μM tested against Taq DNA polymerase. Compound 11 (per-O-acetyl-verbascoside) was the most active with IC50 of 1.21 ± 0.18μM; Compound 9, 2 and 8 are strong inhibitors with IC50 values ​​of 5:57 ± 0.70, 21.62 ± 0.22 and 78.13 ± 0.93μM, respectively. Compound 11 and 9 could be the structure of a new leader for the development of anticancer drugs chemotherapy and useful tools to investigate the activity of DNA polymerase.


Oriented methods and activity-preserved immobilization of biologically active proteins based on the concept of active-site masking and previously reported kinetic control. We extended our research and found that hydrophobic surfaces have a noticeable effect on the activity of the active-protected site move (PIM) Taq DNA polymerase in a mixture of assembled monolayer (SAM) is produced on the Au surface. Hydrophobic SAM created using 12-mercaptododecanoic acid and 1-heptanethiol. Taq DNA polymerase activity generated is measured by PCR amplification and compared with previously reported values ​​obtained with the hydrophilic SAM. The maximum activity of the immobilized Taq DNA polymerase was achieved in 17.5% of 12-mercaptododecanoic acid and within 90 minutes of reaction time is higher than that obtained with the hydrophilic SAM; The maximum activity by 5% 12-mercaptododecanoic acid and at 10 minutes. To apply to a commercial level, moving enzymes have to be stable, reusable and can be stored.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500
EUR 614.4

Taq DNA Polymerase

9001-500
EUR 229.2

PFU DNA Polymerase

9003-2500
EUR 614.4

PFU DNA Polymerase

9003-500
EUR 216

Laq? DNA Polymerase

9004-2500
EUR 732

Laq? DNA Polymerase

9004-500
EUR 261.6

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

Ready? DNA Polymerase

M1146-1000
EUR 398.4

Ready? DNA Polymerase

M1146-10000
EUR 1279.2

Ready? DNA Polymerase

M1146-5000
EUR 960

Breeze? DNA Polymerase

M1148-1000
EUR 1246.8

Breeze? DNA Polymerase

M1148-250
EUR 464.4

Distant? DNA Polymerase

M1150-1000
EUR 823.2

Distant? DNA Polymerase

M1150-250
EUR 333.6

Advance? DNA Polymerase

M1151-1000
EUR 895.2

Advance? DNA Polymerase

M1151-250
EUR 373.2

Outstretched? DNA Polymerase

M1152-1000
EUR 960

Outstretched? DNA Polymerase

M1152-250
EUR 398.4

T4 DNA Polymerase

M1211-100
EUR 385.2

Phi29 DNA Polymerase

M1239-1000
EUR 373.2

AceTaq DNA Polymerase

P401-d1 250 U
EUR 163.2

AceTaq DNA Polymerase

P401-d2 1000 U
EUR 272.4

AceTaq DNA Polymerase

P401-d3 3000 U
EUR 536.4

Taq DNA Polymerase

L7051001 1000 unit
EUR 234
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 104.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 104.4

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 189.6

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

Recombinant Pfu DNA Polymerase

7-03322 100U Ask for price

Recombinant Pfu DNA Polymerase

7-03323 500U Ask for price

Recombinant Pfu DNA Polymerase

7-03324 2500U Ask for price

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (+dNTPs)

02-021 200U
EUR 214.8
Description: The Pfu DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (+dNTPs)

02-021-5 5×200U
EUR 402
Description: The Pfu DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (-dNTPs)

02-031 200U
EUR 199.2
Description: The Pfu DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Pfu DNA polymerase (-dNTPs)

02-031-5 5×200U
EUR 339.6
Description: The Pfu DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (C-His)

10-105 50ug
EUR 637.2
Description: The DNA polymerase ? (C-His) is available in Europe and for worldwide shipping via Gentaur.

T DNA Polymerase Enzyme

abx071008-250U 250 U
EUR 226.8

T DNA Polymerase Enzyme

abx071008-3kU 3 kU
EUR 543.6

T DNA Polymerase Enzyme

abx071008-500U 500 U
EUR 260.4

TopTaq DNA Polymerase Enzyme

abx071014-250U 250 U
EUR 276

TopTaq DNA Polymerase Enzyme

abx071014-3kU 3 kU
EUR 944.4

TopTaq DNA Polymerase Enzyme

abx071014-500U 500 U
EUR 360

EasyPfu DNA Polymerase Enzyme

abx071016-250U 250 U
EUR 226.8

EasyPfu DNA Polymerase Enzyme

abx071016-3kU 3 kU
EUR 526.8

EasyPfu DNA Polymerase Enzyme

abx071016-500U 500 U
EUR 260.4

FastPfu DNA Polymerase Enzyme

abx071018-250U 250 U
EUR 309.6

FastPfu DNA Polymerase Enzyme

abx071018-3kU 3 kU
EUR 1111.2

FastPfu DNA Polymerase Enzyme

abx071018-500U 500 U
EUR 376.8

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Pfu DNA Polymerase (Recombinant)

abx074017-100U 100 U
EUR 393.6

Pfu DNA Polymerase (Recombinant)

abx074017-2500U 2500 U
EUR 1528.8

Pfu DNA Polymerase (Recombinant)

abx074017-500U 500 U
EUR 526.8

PIM stable Taq DNA polymerase attached to the surface and can be used for as many as 10 runs PCR comparable with enzyme-phase solution. Even after 56 days of storage at 4 ° C, the immobilized enzyme classified as 70% of the initial PIM enzyme activity. These data indicate that the PIM Taq DNA polymerase can be used for a variety of commercial applications.