High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.

A polymerase thermostable DNA which has an associated exonuclease activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus Furiosus (PFU). To test its loyalty, we used a genetic dosage that directly measures the fidelity in vitro polymerase DNA during the reaction of the polymerase chain (PCR). Our results indicate that the PCR carried out with purified DNA polymerase from P. Furiosus provides amplification products containing less than 10% of the number of mutations obtained from similar amplifications made with a DNA Taq polymerase. The Fidelity PCR test is based on the amplification and cloning of Laci, Laco and Lacz Alpha (Lacioz Alpha) gene sequences using the PFU or DNA Taq Polymerase.

Some mutations within the LACI gene inactivate the prosecutor’s lake protein and allow the expression of beta-gal. When plated on a chromogenic substrate, these lacines have a blue-plate phenotype. These studies demonstrate that the error rate by induced nucleotide in the 182 known detectable sites of the LACI gene was 1.6 x 10 (-6) for the PFU DNA polymerase, an improvement greater than ten times on the error rate. 2.0 x 10 (-5) for Taq DNA polymerase, after approx. 10 (5) Amplification. Sequences of two hypervarial regions often contained duplicates or deletions from 3 to 15 to 15 pbs) and by the amplification of the peripheral mononuclear cell DNA containing 10 (2) or 10 (3) proviral molecules and analyzing the product By high resolution electrophoresis, the total number and abundance of distinct-length variants within a person may be estimated, providing a more complete analysis of the variants present than it would be obtained by sequencing alone.

The sequences of many people showed frequent amino acid substitutions at certain key positions for antibody neutralization and cytotoxic recognition of the T cell in the immunodominating loop. Non synonymous and non-synonymous nucleotide substitution rates in the region of this region and the flanking areas indicate that a strong positive selection for the change of amino acid works in the generation of antigenic diversity.

The propeller-helical-propeller DNA connection pattern: a structural base for the recognition of non-sequence specific DNA.

One, two or four copies of the Helix-Helx-Helix-Helix (HHH) DNA binding pattern would take place in 14 families homologous protein. The predicted DNA binding function of this pattern is indicated as being compatible with the crystallographic structure of the polymerase rat beta, complexed with a template-primer of DNA [Pelletier, H., SAWAYA, M., Kumar, A., Wilson, SH and Kraut, J. (1994) Science 264, 1891-1903] and with biochemical data. Five predicted HHH motifs crystalline structures are currently known: two of the POL rat beta and one in endonuclease III, Alka and the 5 ‘of Taq Pol I.

These reasons are more structurally similar to those of all Another structure of current databases, including Helix-Turnix patterns. The consolidation of the five HHH structures separately from other helical research structures indicates that all members of the 14 protein families described here have similar HHH structures. By analogy with the beta structure of RAT pol, it is suggested that each of these motifs HHH binds the DNA non-sequence, via the formation of hydrogen bonds between the tritrogen of the protein and the phosphate groups of DNA.

This type of interaction contrasts with the sequence-specific interactions of other patterns, including Helix-Turnix structures. Additional evidence is provided that the host stop proteins of the Alphaherpesvirus Virion host are members of the 5′-nuclease polymerase gene family and Fen1 type endonuclease, and a new DNA binding domain containing HHH occurs in the head of the Kinesin molecule, and in other proteins such as CNJB, EMC-5 and SPT6.

High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.
High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.

Polarization of fluorescence in homogeneous nucleic acid analysis.

A new diagnostic method of the DNA based on the primer extension directed by models and the detection by the polarization of the fluorescence is described. In this method, the amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize with the adn gabin adjacent to the polymorphic site) in the presence of trichhosphosphates of donoxyribonucleoside labeled specific to the allele and A DNA of Taq Polymerase DNA modified in the trade. . The primer is extended by the specific dye terminator for the template on the template, increasing by about 10 times the molecular weight of the fluorophore. At the end of the reaction, the polarization of the fluorescence of the two dye terminators in the reaction mixture is analyzed directly without separation or purification.

This homogeneous DNA diagnostic method is shown very sensitive and specific and is suitable for automated genotyping of large numbers of samples. Recently determined structures of reverse transcriptase and transcriptase and TAQ polymerase with a DNA primer and an incoming nucleotide have shown that a significant conformation change configures the active polymerase site for nucleotidyl transfer . The structure of the reverse transcriptase in the catalytic complex will open the way to the rational design of new analog nucleoside inhibitors of viral replication. A two-step protocol for the extraction and purification of total DNA soil samples has been developed. The raw DNA extracts (100 microliters of 5 g of soil) were contaminated with humic acids at concentrations of 0.7 to 3.3 micrograms / micro-manifests, depending on the type of extracted soil.

DNA Polymerase Iota (POLI) Antibody

abx036752-100ug 100 ug
EUR 469.2

DNA Polymerase Iota (POLI) Antibody

20-abx333843
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

20-abx323859
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 577.2

DNA Polymerase iota Antibody / POLI

RQ4298 100 ug
EUR 419

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase iota

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 352.8

DNA polymerase iota (POLI) polyclonal antibody

ABP-PAB-11630 100 ug Ask for price

DNA Polymerase Iota (POLI) Antibody (HRP)

20-abx335403
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (FITC)

20-abx335404
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (Biotin)

20-abx335405
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human DNA Polymerase Iota (POLI) ELISA Kit

abx382346-96tests 96 tests
EUR 1093.2

Human DNA polymerase iota, POLI ELISA KIT

ELI-38100h 96 Tests
EUR 988.8

Mouse DNA polymerase iota, Poli ELISA KIT

ELI-12742m 96 Tests
EUR 1038

Anti-DNA pol iota Antibody

A01376 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for DNA pol iota Antibody (POLI) detection. Tested with WB in Human.

Anti-DNA pol iota antibody

STJ92742 200 µl
EUR 236.4
Description: Rabbit polyclonal to DNA pol iota.

DNA Polymerase

ABF5235 100 ug
EUR 525.6

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

9001-2500
EUR 614.4

Taq DNA Polymerase

9001-500
EUR 229.2

PFU DNA Polymerase

9003-2500
EUR 614.4

PFU DNA Polymerase

9003-500
EUR 216

Laq? DNA Polymerase

9004-2500
EUR 732

Laq? DNA Polymerase

9004-500
EUR 261.6

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Ready? DNA Polymerase

M1146-1000
EUR 398.4

Ready? DNA Polymerase

M1146-10000
EUR 1279.2

Ready? DNA Polymerase

M1146-5000
EUR 960

Breeze? DNA Polymerase

M1148-1000
EUR 1246.8

Breeze? DNA Polymerase

M1148-250
EUR 464.4

Distant? DNA Polymerase

M1150-1000
EUR 823.2

Distant? DNA Polymerase

M1150-250
EUR 333.6

Advance? DNA Polymerase

M1151-1000
EUR 895.2

Advance? DNA Polymerase

M1151-250
EUR 373.2

Outstretched? DNA Polymerase

M1152-1000
EUR 960

Outstretched? DNA Polymerase

M1152-250
EUR 398.4

T4 DNA Polymerase

M1211-100
EUR 385.2

Phi29 DNA Polymerase

M1239-1000
EUR 373.2

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

Taq DNA Polymerase

L7051001 1000 unit
EUR 234
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 104.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 104.4

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 189.6

AceTaq DNA Polymerase

P401-d1 250 U
EUR 163.2

AceTaq DNA Polymerase

P401-d2 1000 U
EUR 272.4

AceTaq DNA Polymerase

P401-d3 3000 U
EUR 536.4

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

PKC Iota (PKC iota) Antibody

abx033120-400ul 400 ul
EUR 627.6

PKC Iota (PKC iota) Antibody

abx033120-80l 80 µl
EUR 343.2

PKC Iota (PKC iota) Antibody

abx236486-100ug 100 ug
EUR 577.2

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Taq DNA Polymerase Polyclonal Antibody

7018-100
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T
EUR 175.2

DNA polymerase delta cat antibody

70R-12690 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase delta cat antibody

Anti-DNA Polymerase thet Antibody

A02205 100ul
EUR 476.4
Description: Rabbit Polyclonal DNA Polymerase thet Antibody. Validated in IHC and tested in Human.

DNA Polymerase alpha 1 antibody

70R-50225 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase alpha 1 antibody

DNA Polymerase delta 1 antibody

70R-50227 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase delta 1 antibody

DNA Polymerase epsilon 2 antibody

70R-50228 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase epsilon 2 antibody

DNA Polymerase delta 3 antibody

70R-50825 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase delta 3 antibody

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Human DNA Polymerase beta Antibody

33292-05111 150 ug
EUR 313.2

DNA Polymerase Mu (POLM) Antibody

20-abx141146
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Zeta (REV3L) Antibody

20-abx133309
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx004686
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Lambda (POLL) Antibody

20-abx005922
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

The cooling humic acid fraction of a clay silt was similar to that of a commercially available standard humic acid mixture, as determined by electrophoretic mobility in agarose gels, UV fluorescence and Inhibition dosages with enzymes transforming DNA. Restriction endonucleases were inhibited at concentrations of humic acid from 0.5 to 17.2 micrograms / ml for commercial product and from 0.8 to 51.7 micrograms / ml for coextraits humic acids.