High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.

A polymerase thermostable DNA which has an associated exonuclease activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus Furiosus (PFU). To test its loyalty, we used a genetic dosage that directly measures the fidelity in vitro polymerase DNA during the reaction of the polymerase chain (PCR). Our results indicate that the PCR carried out with purified DNA polymerase from P. Furiosus provides amplification products containing less than 10% of the number of mutations obtained from similar amplifications made with a DNA Taq polymerase. The Fidelity PCR test is based on the amplification and cloning of Laci, Laco and Lacz Alpha (Lacioz Alpha) gene sequences using the PFU or DNA Taq Polymerase.

Some mutations within the LACI gene inactivate the prosecutor’s lake protein and allow the expression of beta-gal. When plated on a chromogenic substrate, these lacines have a blue-plate phenotype. These studies demonstrate that the error rate by induced nucleotide in the 182 known detectable sites of the LACI gene was 1.6 x 10 (-6) for the PFU DNA polymerase, an improvement greater than ten times on the error rate. 2.0 x 10 (-5) for Taq DNA polymerase, after approx. 10 (5) Amplification. Sequences of two hypervarial regions often contained duplicates or deletions from 3 to 15 to 15 pbs) and by the amplification of the peripheral mononuclear cell DNA containing 10 (2) or 10 (3) proviral molecules and analyzing the product By high resolution electrophoresis, the total number and abundance of distinct-length variants within a person may be estimated, providing a more complete analysis of the variants present than it would be obtained by sequencing alone.

The sequences of many people showed frequent amino acid substitutions at certain key positions for antibody neutralization and cytotoxic recognition of the T cell in the immunodominating loop. Non synonymous and non-synonymous nucleotide substitution rates in the region of this region and the flanking areas indicate that a strong positive selection for the change of amino acid works in the generation of antigenic diversity.

The propeller-helical-propeller DNA connection pattern: a structural base for the recognition of non-sequence specific DNA.

One, two or four copies of the Helix-Helx-Helix-Helix (HHH) DNA binding pattern would take place in 14 families homologous protein. The predicted DNA binding function of this pattern is indicated as being compatible with the crystallographic structure of the polymerase rat beta, complexed with a template-primer of DNA [Pelletier, H., SAWAYA, M., Kumar, A., Wilson, SH and Kraut, J. (1994) Science 264, 1891-1903] and with biochemical data. Five predicted HHH motifs crystalline structures are currently known: two of the POL rat beta and one in endonuclease III, Alka and the 5 ‘of Taq Pol I.

These reasons are more structurally similar to those of all Another structure of current databases, including Helix-Turnix patterns. The consolidation of the five HHH structures separately from other helical research structures indicates that all members of the 14 protein families described here have similar HHH structures. By analogy with the beta structure of RAT pol, it is suggested that each of these motifs HHH binds the DNA non-sequence, via the formation of hydrogen bonds between the tritrogen of the protein and the phosphate groups of DNA.

This type of interaction contrasts with the sequence-specific interactions of other patterns, including Helix-Turnix structures. Additional evidence is provided that the host stop proteins of the Alphaherpesvirus Virion host are members of the 5′-nuclease polymerase gene family and Fen1 type endonuclease, and a new DNA binding domain containing HHH occurs in the head of the Kinesin molecule, and in other proteins such as CNJB, EMC-5 and SPT6.

High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.
High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.

Polarization of fluorescence in homogeneous nucleic acid analysis.

A new diagnostic method of the DNA based on the primer extension directed by models and the detection by the polarization of the fluorescence is described. In this method, the amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize with the adn gabin adjacent to the polymorphic site) in the presence of trichhosphosphates of donoxyribonucleoside labeled specific to the allele and A DNA of Taq Polymerase DNA modified in the trade. . The primer is extended by the specific dye terminator for the template on the template, increasing by about 10 times the molecular weight of the fluorophore. At the end of the reaction, the polarization of the fluorescence of the two dye terminators in the reaction mixture is analyzed directly without separation or purification.

This homogeneous DNA diagnostic method is shown very sensitive and specific and is suitable for automated genotyping of large numbers of samples. Recently determined structures of reverse transcriptase and transcriptase and TAQ polymerase with a DNA primer and an incoming nucleotide have shown that a significant conformation change configures the active polymerase site for nucleotidyl transfer . The structure of the reverse transcriptase in the catalytic complex will open the way to the rational design of new analog nucleoside inhibitors of viral replication. A two-step protocol for the extraction and purification of total DNA soil samples has been developed. The raw DNA extracts (100 microliters of 5 g of soil) were contaminated with humic acids at concentrations of 0.7 to 3.3 micrograms / micro-manifests, depending on the type of extracted soil.

DNA Polymerase Iota (POLI) Antibody

abx028115-80l 80 µl
EUR 343.2

DNA Polymerase Iota (POLI) Antibody

20-abx001585
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  • 100 ul
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DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 577.2

DNA Polymerase Iota (POLI) Antibody

20-abx333843
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  • 100 ug
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DNA Polymerase Iota (POLI) Antibody

20-abx323859
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  • 100 ug
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DNA Polymerase Iota (POLI) Antibody

abx001585-100l 100 µl
EUR 400

DNA Polymerase Iota (POLI) Antibody

abx001585-20l 20 µl
EUR 175

DNA Polymerase Iota (POLI) Antibody

abx001585-50l 50 µl
EUR 275

DNA Polymerase Iota (POLI) Antibody

abx036752-100g 100 µg
EUR 337.5

DNA Polymerase Iota (POLI) Antibody

abx028115-400l 400 µl
EUR 518.75

DNA Polymerase Iota (POLI) Antibody

abx236612-100g 100 µg
EUR 350

DNA Polymerase Iota (POLI) Antibody

abx333843-100g 100 µg
EUR 362.5

DNA Polymerase Iota (POLI) Antibody

abx333843-20g 20 µg
EUR 162.5

DNA Polymerase Iota (POLI) Antibody

abx333843-50g 50 µg
EUR 250

DNA Polymerase Iota (POLI) Antibody

abx323859-100g 100 µg
EUR 250

DNA Polymerase Iota (POLI) Antibody

abx323859-50g 50 µg
EUR 187.5

Polymerase (Dna Directed) iota Antibody

20-abx114586
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  • 150 ul
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Polymerase (Dna Directed) iota Antibody

abx114586-100l 100 µl
EUR 612.5

Rabbit Polyclonal DNA Polymerase iota Antibody

TA309813 50 µl Ask for price

DNA Polymerase Iota (POLI) Antibody (HRP)

20-abx335403
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  • 100 ug
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DNA Polymerase Iota (POLI) Antibody (HRP)

abx335403-100g 100 µg
EUR 362.5

DNA Polymerase Iota (POLI) Antibody (HRP)

abx335403-20g 20 µg
EUR 162.5

DNA Polymerase Iota (POLI) Antibody (HRP)

abx335403-50g 50 µg
EUR 250

DNA Polymerase Iota (POLI) Antibody (FITC)

20-abx335404
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DNA Polymerase Iota (POLI) Antibody (FITC)

abx335404-100g 100 µg
EUR 362.5

DNA Polymerase Iota (POLI) Antibody (FITC)

abx335404-20g 20 µg
EUR 162.5

DNA Polymerase Iota (POLI) Antibody (FITC)

abx335404-50g 50 µg
EUR 250

DNA Polymerase Iota (POLI) Antibody (Biotin)

20-abx335405
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  • 100 ug
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DNA Polymerase Iota (POLI) Antibody (Biotin)

abx335405-100g 100 µg
EUR 362.5

DNA Polymerase Iota (POLI) Antibody (Biotin)

abx335405-20g 20 µg
EUR 162.5

DNA Polymerase Iota (POLI) Antibody (Biotin)

abx335405-50g 50 µg
EUR 250

DNA polymerase iota (POLI) polyclonal antibody

ABP-PAB-11630 100 ug Ask for price

Rabbit anti-DNA Polymerase iota Antibody

DL94564A-100ul 100 ul
EUR 289
Description: RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

Rabbit anti-DNA Polymerase iota Antibody

DL94564A-50ul 50 ul
EUR 202.3
Description: RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

Rabbit anti-DNA Polymerase iota Antibody

YLD2560-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to DNA Polymerase iota

Rabbit anti-DNA Polymerase iota Antibody

YLD2560-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to DNA Polymerase iota

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 352.8

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase iota

Human DNA Polymerase Iota (POLI) ELISA Kit

abx382346-96tests 96 tests
EUR 1093.2

Human DNA polymerase iota, POLI ELISA KIT

ELI-38100h 96 Tests
EUR 988.8

Mouse DNA polymerase iota, Poli ELISA KIT

ELI-12742m 96 Tests
EUR 1038

Mouse DNA polymerase iota (POLI) ELISA Kit

abx539525-96tests 96 tests
EUR 687.5

DNA Polymerase iota (POLI) (N-term) rabbit polyclonal antibody, Aff - Purified

AP46109PU-N 100 µl Ask for price

POLI (untagged)-Human polymerase (DNA directed) iota (POLI)

SC318221 10 µg Ask for price

POLI (untagged)-Human polymerase (DNA directed) iota (POLI)

SC318222 10 µg Ask for price

POLI (GFP-tagged) - Human polymerase (DNA directed) iota (POLI)

RG207550 10 µg Ask for price

POLI (Myc-DDK-tagged)-Human polymerase (DNA directed) iota (POLI)

RC207550 10 µg Ask for price

Poli (untagged ORF) - Rat polymerase (DNA directed), iota (Poli), (10 ug)

RN204969 10 µg Ask for price

Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), mGFP tagged

RC207550L2 10 µg Ask for price

Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), mGFP tagged

RC207550L4 10 µg Ask for price

Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 3

MC228259 10 µg Ask for price

Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 4

MC228581 10 µg Ask for price

Poli (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), iota (Poli), (10 ug)

RR204969 10 µg Ask for price

DNA Polymerase Antibody

8C10144 50ug
EUR 368
Description: DNA Polymerase ζ Antibody

DNA Polymerase Antibody

E11-11836C 100μg
EUR 225
Description: Available in various conjugation types.

Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 1, (10ug)

MC220331 10 µg Ask for price

Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2, (10ug)

MC221231 10 µg Ask for price

Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), Myc-DDK-tagged

RC207550L1 10 µg Ask for price

Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), Myc-DDK-tagged

RC207550L3 10 µg Ask for price

Poli (GFP-tagged) - Mouse polymerase (DNA directed), iota (cDNA clone MGC:91286 IMAGE:6328261)

MG209951 10 µg Ask for price

Poli (myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 3

MR230470 10 µg Ask for price

Poli (myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 4

MR230792 10 µg Ask for price

Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 1

MR209951 10 µg Ask for price

Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2

MR216260 10 µg Ask for price

Poli (GFP-tagged) - Mouse polymerase (DNA directed) iota (Poli) transcript variant 2, (10ug)

MG216260 10 µg Ask for price

DNA Polymerase β Antibody

8C0173 50ug
EUR 368
Description: DNA Polymerase β Antibody

DNA Polymerase θ Antibody

8C10173 50ug
EUR 368
Description: DNA Polymerase θ Antibody

DNA Polymerase α Antibody

8C10322 50ug
EUR 368
Description: DNA Polymerase α Antibody

DNA Polymerase λ Antibody

8C11836 50ug
EUR 368
Description: DNA Polymerase λ Antibody

DNA Polymerase β Antibody

33357 100ul
EUR 319

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602 100ul
EUR 319

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611 100ul
EUR 319

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665 100ul
EUR 319

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E033602 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E11-10144C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

20-abx013060
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  • 100 ug
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DNA Polymerase beta Antibody

20-abx008852
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DNA Polymerase beta Antibody

35404 100ul
EUR 479

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

49605 100ul
EUR 499

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

E18-0148-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

E18-0148-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

DF6552-200ul 200ul
EUR 350

DNA Polymerase zeta Antibody

E38PA4807 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 342
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 242
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

AF0148-200ul 200ul
EUR 350

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

GWB-651A32 0.5 ml Ask for price

DNA polymerase beta Antibody

GWB-C8D577 0.25 ml Ask for price

DNA Polymerase beta Antibody

abx013060-100g 100 µg
EUR 237.5

DNA Polymerase beta Antibody

abx013060-10g 10 µg
EUR 43.75

DNA Polymerase beta Antibody

abx013060-200g 200 µg
EUR 312.5

DNA polymerase beta Antibody

abx232432-100g 100 µg
EUR 350

DNA Polymerase alpha Antibody

20-abx013375
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  • 100 ug
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DNA Polymerase gamma antibody

22870 100ul
EUR 479

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase lamda Antibody

34098 100ul
EUR 319

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase theta Antibody

DF13563-100ul 100ul
EUR 280

DNA Polymerase theta Antibody

DF13563-200ul 200ul
EUR 350

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase alpha Antibody

DF3082-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

DF3082-200ul 200ul
EUR 350

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase alpha Antibody

AF5235-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

AF5235-200ul 200ul
EUR 350

DNA polymerase delta Antibody

GWB-86F322 0.25 mg Ask for price

DNA polymerase delta Antibody

GWB-7399F1 0.05 ml Ask for price

DNA polymerase delta Antibody

GWB-C04C62 0.1 mg Ask for price

DNA Polymerase alpha Antibody

abx013375-100g 100 µg
EUR 237.5

The cooling humic acid fraction of a clay silt was similar to that of a commercially available standard humic acid mixture, as determined by electrophoretic mobility in agarose gels, UV fluorescence and Inhibition dosages with enzymes transforming DNA. Restriction endonucleases were inhibited at concentrations of humic acid from 0.5 to 17.2 micrograms / ml for commercial product and from 0.8 to 51.7 micrograms / ml for coextraits humic acids.