High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.

A polymerase thermostable DNA which has an associated exonuclease activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus Furiosus (PFU). To test its loyalty, we used a genetic dosage that directly measures the fidelity in vitro polymerase DNA during the reaction of the polymerase chain (PCR). Our results indicate that the PCR carried out with purified DNA polymerase from P. Furiosus provides amplification products containing less than 10% of the number of mutations obtained from similar amplifications made with a DNA Taq polymerase. The Fidelity PCR test is based on the amplification and cloning of Laci, Laco and Lacz Alpha (Lacioz Alpha) gene sequences using the PFU or DNA Taq Polymerase.

Some mutations within the LACI gene inactivate the prosecutor’s lake protein and allow the expression of beta-gal. When plated on a chromogenic substrate, these lacines have a blue-plate phenotype. These studies demonstrate that the error rate by induced nucleotide in the 182 known detectable sites of the LACI gene was 1.6 x 10 (-6) for the PFU DNA polymerase, an improvement greater than ten times on the error rate. 2.0 x 10 (-5) for Taq DNA polymerase, after approx. 10 (5) Amplification. Sequences of two hypervarial regions often contained duplicates or deletions from 3 to 15 to 15 pbs) and by the amplification of the peripheral mononuclear cell DNA containing 10 (2) or 10 (3) proviral molecules and analyzing the product By high resolution electrophoresis, the total number and abundance of distinct-length variants within a person may be estimated, providing a more complete analysis of the variants present than it would be obtained by sequencing alone.

The sequences of many people showed frequent amino acid substitutions at certain key positions for antibody neutralization and cytotoxic recognition of the T cell in the immunodominating loop. Non synonymous and non-synonymous nucleotide substitution rates in the region of this region and the flanking areas indicate that a strong positive selection for the change of amino acid works in the generation of antigenic diversity.

The propeller-helical-propeller DNA connection pattern: a structural base for the recognition of non-sequence specific DNA.

One, two or four copies of the Helix-Helx-Helix-Helix (HHH) DNA binding pattern would take place in 14 families homologous protein. The predicted DNA binding function of this pattern is indicated as being compatible with the crystallographic structure of the polymerase rat beta, complexed with a template-primer of DNA [Pelletier, H., SAWAYA, M., Kumar, A., Wilson, SH and Kraut, J. (1994) Science 264, 1891-1903] and with biochemical data. Five predicted HHH motifs crystalline structures are currently known: two of the POL rat beta and one in endonuclease III, Alka and the 5 ‘of Taq Pol I.

These reasons are more structurally similar to those of all Another structure of current databases, including Helix-Turnix patterns. The consolidation of the five HHH structures separately from other helical research structures indicates that all members of the 14 protein families described here have similar HHH structures. By analogy with the beta structure of RAT pol, it is suggested that each of these motifs HHH binds the DNA non-sequence, via the formation of hydrogen bonds between the tritrogen of the protein and the phosphate groups of DNA.

This type of interaction contrasts with the sequence-specific interactions of other patterns, including Helix-Turnix structures. Additional evidence is provided that the host stop proteins of the Alphaherpesvirus Virion host are members of the 5′-nuclease polymerase gene family and Fen1 type endonuclease, and a new DNA binding domain containing HHH occurs in the head of the Kinesin molecule, and in other proteins such as CNJB, EMC-5 and SPT6.

High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.
High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase.

Polarization of fluorescence in homogeneous nucleic acid analysis.

A new diagnostic method of the DNA based on the primer extension directed by models and the detection by the polarization of the fluorescence is described. In this method, the amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize with the adn gabin adjacent to the polymorphic site) in the presence of trichhosphosphates of donoxyribonucleoside labeled specific to the allele and A DNA of Taq Polymerase DNA modified in the trade. . The primer is extended by the specific dye terminator for the template on the template, increasing by about 10 times the molecular weight of the fluorophore. At the end of the reaction, the polarization of the fluorescence of the two dye terminators in the reaction mixture is analyzed directly without separation or purification.

This homogeneous DNA diagnostic method is shown very sensitive and specific and is suitable for automated genotyping of large numbers of samples. Recently determined structures of reverse transcriptase and transcriptase and TAQ polymerase with a DNA primer and an incoming nucleotide have shown that a significant conformation change configures the active polymerase site for nucleotidyl transfer . The structure of the reverse transcriptase in the catalytic complex will open the way to the rational design of new analog nucleoside inhibitors of viral replication. A two-step protocol for the extraction and purification of total DNA soil samples has been developed. The raw DNA extracts (100 microliters of 5 g of soil) were contaminated with humic acids at concentrations of 0.7 to 3.3 micrograms / micro-manifests, depending on the type of extracted soil.

DNA Polymerase Iota (POLI) Antibody

abx028115-400ul 400 ul
EUR 523

DNA Polymerase Iota (POLI) Antibody

abx028115-80l 80 µl
EUR 286

DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 481

DNA Polymerase Iota (POLI) Antibody

20-abx333843
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase iota

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 294

DNA Polymerase Iota (POLI) Antibody (HRP)

20-abx335403
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (FITC)

20-abx335404
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody (Biotin)

20-abx335405
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA polymerase iota (POLI) polyclonal antibody

ABP-PAB-11630 100 ug Ask for price

Human DNA Polymerase Iota (POLI) ELISA Kit

abx382346-96tests 96 tests
EUR 911

Human DNA polymerase iota, POLI ELISA KIT

ELI-38100h 96 Tests
EUR 824

Mouse DNA polymerase iota, Poli ELISA KIT

ELI-12742m 96 Tests
EUR 865

Anti-DNA pol iota Antibody

A01376 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for DNA pol iota Antibody (POLI) detection. Tested with WB in Human.

Anti-DNA pol iota antibody

STJ92742 200 µl
EUR 197
Description: Rabbit polyclonal to DNA pol iota.

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 390

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 390

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 252

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 187

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 252

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 187

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 252

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 187

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 252

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 187

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 252

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 187

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 333

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 239

DNA Polymerase beta Antibody

20-abx008852
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 481

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 438

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 304
Description: DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta.

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 244
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase theta Antibody

DF13563 100ul
EUR 350

PKC Iota (PKC iota) Antibody

abx033120-400ul 400 ul
EUR 523

PKC Iota (PKC iota) Antibody

abx033120-80l 80 µl
EUR 286

PKC Iota (PKC iota) Antibody

abx236486-100ug 100 ug
EUR 481

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

S116 1x250 units
EUR 76

Pfu DNA Polymerase

S117 2x250 units
EUR 94

Pfu DNA Polymerase

S118 10x250 units
EUR 303

Bst DNA Polymerase

S600 2000 U
EUR 107

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 145
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 239
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 522
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 123
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 196
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 413
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 145
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 239
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 522
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 120
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 190
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 87

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 158

Taq DNA Polymerase

L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Ready? DNA Polymerase

M1146-1000
EUR 332

Ready? DNA Polymerase

M1146-10000
EUR 1066

Ready? DNA Polymerase

M1146-5000
EUR 800

Breeze? DNA Polymerase

M1148-1000
EUR 1039

Breeze? DNA Polymerase

M1148-250
EUR 387

Distant? DNA Polymerase

M1150-1000
EUR 686

Distant? DNA Polymerase

M1150-250
EUR 278

Advance? DNA Polymerase

M1151-1000
EUR 746

Advance? DNA Polymerase

M1151-250
EUR 311

Outstretched? DNA Polymerase

M1152-1000
EUR 800

Outstretched? DNA Polymerase

M1152-250
EUR 332

T4 DNA Polymerase

M1211-100
EUR 321

Phi29 DNA Polymerase

M1239-1000
EUR 311

T4 DNA Polymerase

N101-01 2000 U
EUR 232

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

PFU DNA Polymerase

9003-2500
EUR 512

PFU DNA Polymerase

9003-500
EUR 180

Laq? DNA Polymerase

9004-2500
EUR 610

Laq? DNA Polymerase

9004-500
EUR 218

AceTaq DNA Polymerase

P401-d1 250 U
EUR 136

AceTaq DNA Polymerase

P401-d2 1000 U
EUR 227

AceTaq DNA Polymerase

P401-d3 3000 U
EUR 447

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Human DNA Polymerase beta Antibody

33292-05111 150 ug
EUR 261

Anti-DNA polymerase eta Antibody

STJ502747 100 µg
EUR 476

DNA Polymerase Kappa (POLK) Antibody

20-abx126383
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx126384
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx126385
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Eta (POLH) Antibody

abx117166-100ug 100 ug
EUR 467

Polymerase (Dna Directed) kappa Antibody

20-abx114587
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Polymerase (Dna Directed), beta Antibody

20-abx114589
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Polymerase (Dna Directed), Mu Antibody

20-abx114594
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

DNA polymerase I, thermostable Antibody

20-abx110639
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Theta (POLQ) Antibody

20-abx322965
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

DNA Polymerase Mu (POLM) Antibody

20-abx323923
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

DNA Polymerase Kappa (POLK) Antibody

20-abx338646
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Eta (POLH) Antibody

20-abx001515
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx004686
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Lambda (POLL) Antibody

abx034579-400ul 400 ul
EUR 523

DNA Polymerase Lambda (POLL) Antibody

abx034579-80l 80 µl
EUR 286

The cooling humic acid fraction of a clay silt was similar to that of a commercially available standard humic acid mixture, as determined by electrophoretic mobility in agarose gels, UV fluorescence and Inhibition dosages with enzymes transforming DNA. Restriction endonucleases were inhibited at concentrations of humic acid from 0.5 to 17.2 micrograms / ml for commercial product and from 0.8 to 51.7 micrograms / ml for coextraits humic acids.