CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.

The genetic diversity of a vesicular stomatitis virus population was analyzed by RT-PCR, cloning and sequencing of about 500 nucleotide regions of the virus genome. PCR amplifications were performed in parallel experiments with DNA Taq and PFU polymerases and significant differences were observed. Between 10 and 22 mutations were detected when virus populations were analyzed by TAQ amplification (20 clones of each region), while the amplification of the same samples with PFU revealed between 0 and 5 mutations. PCR loyalty tests, carried out under the same PCR conditions as those used in the population analysis, have shown that the Taq error rate estimation of 0.27 x 10 (-4) Enporations by PB by Cycle was included in the range estimated elsewhere of the amplification of the recombinant PCR plasmids (0.27-0.85 x 10 (-4) Errors by BP per cycle) or functional tests (0.2-2 x 10 ( -4) Errors by PB per cycle).

The TAQ error rate has been found to be 9.3 times higher than the PFU error rate with DNA as a model, and about 10 times higher with the CDNCs obtained by inverse transcription of models. Viral RNA from natural populations. In this study, we discuss (i) the implications of TAQ errors on the analysis of genetic variability, based on both frequency and nature (synonymous replacement) of the substitutions observed and (ii) the size of the Sample required to evaluate genetics variability in a virus population generated by a single infection. In this project, a real-time polymerase chain reaction (PCR) was used to study the mechanism of PCR inhibition by examining the effect of the amplicon length, the melting temperature and of the sequence.

First specially designed with three different amplicon lengths and three different melting temperatures have been used to target a single homozygous allele in the Humert01 locus. The effect on the efficiency of the amplification of each pair of primers has been determined by adding different concentrations of various PCR inhibitors to the reaction mixture. The results show that various inhibition mechanisms can occur during the PCR process based on the type of co-extract inhibitor. These include TAQ inhibition, the binding of DNA templates and the effects on the efficiency of the reaction.

PCR-resistant PCR-resistant Taq DNA mutants allow for amplification of total blood DNA and gross soil samples.

Powerful PCR inhibitors in blood and soil samples can cause false negative results of PCR-based clinical and lawy tests. We show that the effect of these inhibitors is mainly on the TAQ DNA polymerase, because the mutational modification of the polymerase can overcome the inhibition insofar as no purification of the DNA is now required. N-terminal removal (Klentaq1) is an inhibition of 10 to 100 times resistant to total blood relative to the Taq Wild Type TAQ (W.T.) Taq, which is strongly inhibited by 0.1 to 1% blood. Other mutations at Codon 708, both in Klentaq 1 and Taq, give increased resistance to various PCR reaction inhibitors, including total blood, plasma, hemoglobin, lactoferrin, IgG. Serum, soil extracts and humic acid, as well as high concentrations of inserted dyes.

The blood PCR inhibitors can mainly reduce the speed of extension of the W.T DNA Taq polymerase with respect to mutant enzymes. Human genomic targets to a human copy are easily amplified from total blood or raw soil extract, without pretreatment to purify the template DNA, and the permitted increase in the dye concentration overcomes the background of Fluorescence and quenching in real-time blood PCR.

CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.
CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.

DNA removal contaminant in the reaction reagents of the polymerase chain: implications for a general approach to the detection of unutaneous pathogens.

The analysis based on comparisons of RNA 16S sequences provides a quick and reliable approach to identifying human pathogens. By leading the primers of oligonucleotides to the sequences preserved throughout the kingdom of ebacterial, the bacterial ribosomal DNA sequences of substantially any member of the kingdom of the ebacteria can be amplified by the reaction of the chain of the polymerase and subsequently analyzed by the determination of the sequence. Indeed, automated wide range amplification systems, sequencing and data analysis are now feasible and can be founded on the next generation of automated microbial identification systems.

However, the identification of the pathogens by this strategy is hampered by the frequent contamination of the reagents used for the amplification reaction, in particular the Taq polymerase, with an exogenous bacterial DNA. Here, we describe detailed surveys of the use of 8-methoxypsorane UV light and the long waves to remove the contaminant DNA in the reaction reagents of the polymerase chain. The clinical utility of the developed procedure has been demonstrated in a case of pacifacillary osteomyelitis for which no specific bacterial agent had been cultivated. We have developed and characterized a test for the interactive compounds of G-quadruplex that use the fact that G-rich DNA models have barriers to the synthesis of DNA by DNA polymerases.

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-100ug

QP12758-100ug 100ug
EUR 261.6

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-500ug

QP12758-500ug 500ug
EUR 795.6

Recombinant (E.Coli) purified Measles virus Large Polymerase (58-149)

RP-1613 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 58-149)

VAng-Lsx0397-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 58-149), recombinant protein from E. coli.

Recombinant MeV Large Polymerase Protein

VAng-Lsx0385-inquire inquire Ask for price
Description: Recombinant MeV Large Polymerase containing the large polymerase immunodominant regions, 58-149 amino acids was expressed in E. coli and purified by proprietary chromatographic technique.

Bst DNA Polymerase Large Fragment

P701-01 800 U
EUR 146.4

Bst DNA Polymerase Large Fragment

P701-02 8000 U
EUR 325.2

Bst DNA Polymerase, Large Fragment

M1213-200 each
EUR 346.8

Bsu DNA Polymerase Large Fragment

M1214-200 each
EUR 477.6

Measles Virus Large Polymerase (2059-2183) Protein

20-abx260297
  • EUR 1062.00
  • EUR 410.40
  • EUR 1930.80
  • 0.5 mg
  • 100 ug
  • 1 mg

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07843 100µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07844 500µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07845 1000µg Ask for price

Bst II DNA Polymerase Large Fragment

P702-01 1,600 U
EUR 22

DNA Polymerase I Large (Klenow) Fragment

M1212-100 each
EUR 333.6

Recombinant (E.Coli) Measles Virus Large Polymerase (2059-2183)

RP-653 100 ug
EUR 343.2

Bst II Pro DNA Polymerase Large Fragment

P703-01 1,600 U
EUR 29.39

Bst II Pro DNA Polymerase Large Fragment

P703-02 8,000 U
EUR 116

Recombinant (E.Coli) purified Measles virus Large Polymerase (2059-2183)

RP-1612 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 2059-2183)

VAng-Lsx0399-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 2059-2183), recombinant protein from E. coli.

DNA Polymerase I, Large (Klenow) Fragment - 1,000 units

3262 1/EA
EUR 346.8

Recombinant MeV Polymerase Protein (aa 58-149)

VAng-Lsx0394-inquire inquire Ask for price
Description: MeV Polymerase (aa 58-149), recombinant protein from E. coli.

Cenarchaeum symbiosum DNA polymerase II large subunit (polC)

1-CSB-EP370095DRA
  • EUR 745.20
  • EUR 457.20
  • EUR 2331.60
  • EUR 1058.40
  • EUR 1602.00
  • EUR 541.20
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial expressed in E.coli

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

HBV polymerase (455 - 463)

5-01273 4 x 5mg Ask for price

T7 RNA Polymerase (5000U)

9K-005-0004 5000U
EUR 316.82

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

T4 DNA Polymerase

M1211-100 each
EUR 385.2

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

SP6 RNA Polymerase (5000U)

9K-005-0003 5000U
EUR 462.47

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

PFU DNA Polymerase

9003-2500 each
EUR 614.4

PFU DNA Polymerase

9003-500 each
EUR 216

Laq? DNA Polymerase

9004-2500 each
EUR 732

Laq? DNA Polymerase

9004-500 each
EUR 261.6

Taq DNA Polymerase

L7051001 1000 unit
EUR 159

Taq DNA Polymerase

L7051200 200 unit
EUR 41

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

Ready? DNA Polymerase

M1146-1000 each
EUR 398.4

Ready? DNA Polymerase

M1146-10000 each
EUR 1279.2

Ready? DNA Polymerase

M1146-5000 each
EUR 960

Phi29 DNA Polymerase

M1239-1000 each
EUR 373.2

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 104.4

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 189.6

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

AceTaq DNA Polymerase

P401-d1 250 U
EUR 163.2

AceTaq DNA Polymerase

P401-d2 1000 U
EUR 272.4

AceTaq DNA Polymerase

P401-d3 3000 U
EUR 536.4

Breeze? DNA Polymerase

M1148-1000 each
EUR 1246.8

Breeze? DNA Polymerase

M1148-250 each
EUR 464.4

AceTaq DNA Polymerase

P401-MD1 250 U
EUR 46.4

Distant? DNA Polymerase

M1150-1000 each
EUR 823.2

Distant? DNA Polymerase

M1150-250 each
EUR 333.6

Advance? DNA Polymerase

M1151-1000 each
EUR 895.2

Advance? DNA Polymerase

M1151-250 each
EUR 373.2

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

anti-mtRNA polymerase

YF-PA24420 50 ul
EUR 400.8
Description: Mouse polyclonal to mtRNA polymerase

anti-mtRNA polymerase

YF-PA13878 50 ug
EUR 435.6
Description: Mouse polyclonal to mtRNA polymerase

E. coli RNA polymerase Assay Kit Plus (E. coli RNA polymerase included)

RPA100KE 100 assays
EUR 467.15
Description: This product includes all the reagents in E. coli RNA Polymerase Assay Kit (Catalog number RPA100K) plus the enzyme, 33 ul of 100 x E. coli RNAP. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.

Taq HS DNA Polymerase

P132-d1 500 U
EUR 38.28

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 71.34

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 324.8

DNA polymerase ? (C-His)

10-105 50ug
EUR 637.2
Description: The DNA polymerase ? (C-His) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 104.4

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

T4 DNA Polymerase - 500 units

3222 1/EA
EUR 247.2

HBV polymerase (455 463) Peptide

20-abx266100
  • EUR 493.20
  • EUR 794.40
  • EUR 376.80
  • 10 mg
  • 25 mg
  • 5 mg

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Poly(A) Polymerase Yeast

M1215-100 each
EUR 333.6

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

MyTaq HS DNA Polymerase

BIO-21111 250 units Ask for price

MyTaq HS DNA Polymerase

BIO-21111/S Sample Ask for price

MyTaq HS DNA Polymerase

BIO-21112 1000 units Ask for price

Use of Taq DNA polymerase and G-quadruplex 2, 6-diamidoantoanthraquinone bonding BSU-1051, we find that the BSU-1051 leads to improved arrest of the synthesis of the DNA in the presence of K + by stabilizing an intramolecular structure G-quadruplex formed by four repetitions of TTGGGG or TTAGGG in the model pane. The data provides additional evidence that BSU-1051 modules the telomerase activity by stabilizing G-quadruple telomer DNA and point to a polymerase stop test as a sensitive method for screening for interactive agents of G- Quadruplex with potential clinical utility.