CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.

The genetic diversity of a vesicular stomatitis virus population was analyzed by RT-PCR, cloning and sequencing of about 500 nucleotide regions of the virus genome. PCR amplifications were performed in parallel experiments with DNA Taq and PFU polymerases and significant differences were observed. Between 10 and 22 mutations were detected when virus populations were analyzed by TAQ amplification (20 clones of each region), while the amplification of the same samples with PFU revealed between 0 and 5 mutations. PCR loyalty tests, carried out under the same PCR conditions as those used in the population analysis, have shown that the Taq error rate estimation of 0.27 x 10 (-4) Enporations by PB by Cycle was included in the range estimated elsewhere of the amplification of the recombinant PCR plasmids (0.27-0.85 x 10 (-4) Errors by BP per cycle) or functional tests (0.2-2 x 10 ( -4) Errors by PB per cycle).

The TAQ error rate has been found to be 9.3 times higher than the PFU error rate with DNA as a model, and about 10 times higher with the CDNCs obtained by inverse transcription of models. Viral RNA from natural populations. In this study, we discuss (i) the implications of TAQ errors on the analysis of genetic variability, based on both frequency and nature (synonymous replacement) of the substitutions observed and (ii) the size of the Sample required to evaluate genetics variability in a virus population generated by a single infection. In this project, a real-time polymerase chain reaction (PCR) was used to study the mechanism of PCR inhibition by examining the effect of the amplicon length, the melting temperature and of the sequence.

First specially designed with three different amplicon lengths and three different melting temperatures have been used to target a single homozygous allele in the Humert01 locus. The effect on the efficiency of the amplification of each pair of primers has been determined by adding different concentrations of various PCR inhibitors to the reaction mixture. The results show that various inhibition mechanisms can occur during the PCR process based on the type of co-extract inhibitor. These include TAQ inhibition, the binding of DNA templates and the effects on the efficiency of the reaction.

PCR-resistant PCR-resistant Taq DNA mutants allow for amplification of total blood DNA and gross soil samples.

Powerful PCR inhibitors in blood and soil samples can cause false negative results of PCR-based clinical and lawy tests. We show that the effect of these inhibitors is mainly on the TAQ DNA polymerase, because the mutational modification of the polymerase can overcome the inhibition insofar as no purification of the DNA is now required. N-terminal removal (Klentaq1) is an inhibition of 10 to 100 times resistant to total blood relative to the Taq Wild Type TAQ (W.T.) Taq, which is strongly inhibited by 0.1 to 1% blood. Other mutations at Codon 708, both in Klentaq 1 and Taq, give increased resistance to various PCR reaction inhibitors, including total blood, plasma, hemoglobin, lactoferrin, IgG. Serum, soil extracts and humic acid, as well as high concentrations of inserted dyes.

The blood PCR inhibitors can mainly reduce the speed of extension of the W.T DNA Taq polymerase with respect to mutant enzymes. Human genomic targets to a human copy are easily amplified from total blood or raw soil extract, without pretreatment to purify the template DNA, and the permitted increase in the dye concentration overcomes the background of Fluorescence and quenching in real-time blood PCR.

CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.
CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.

DNA removal contaminant in the reaction reagents of the polymerase chain: implications for a general approach to the detection of unutaneous pathogens.

The analysis based on comparisons of RNA 16S sequences provides a quick and reliable approach to identifying human pathogens. By leading the primers of oligonucleotides to the sequences preserved throughout the kingdom of ebacterial, the bacterial ribosomal DNA sequences of substantially any member of the kingdom of the ebacteria can be amplified by the reaction of the chain of the polymerase and subsequently analyzed by the determination of the sequence. Indeed, automated wide range amplification systems, sequencing and data analysis are now feasible and can be founded on the next generation of automated microbial identification systems.

However, the identification of the pathogens by this strategy is hampered by the frequent contamination of the reagents used for the amplification reaction, in particular the Taq polymerase, with an exogenous bacterial DNA. Here, we describe detailed surveys of the use of 8-methoxypsorane UV light and the long waves to remove the contaminant DNA in the reaction reagents of the polymerase chain. The clinical utility of the developed procedure has been demonstrated in a case of pacifacillary osteomyelitis for which no specific bacterial agent had been cultivated. We have developed and characterized a test for the interactive compounds of G-quadruplex that use the fact that G-rich DNA models have barriers to the synthesis of DNA by DNA polymerases.

OPPA01109-100UG - MV Large Polymerase 2059-2183 a.a Protein

OPPA01109-100UG 100ug
EUR 278

Recombinant (E.Coli) Measles Virus Large Polymerase (58-149)

RP-651 100 ug
EUR 343.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-1mg

QP12758-1mg 1mg
EUR 1513.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-100ug

QP12758-100ug 100ug
EUR 261.6

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-500ug

QP12758-500ug 500ug
EUR 795.6

Recombinant (E.Coli) purified Measles virus Large Polymerase (58-149)

RP-1613 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 58-149)

VAng-Lsx0397-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 58-149), recombinant protein from E. coli.

Recombinant MeV Large Polymerase Protein

VAng-Lsx0385-inquire inquire Ask for price
Description: Recombinant MeV Large Polymerase containing the large polymerase immunodominant regions, 58-149 amino acids was expressed in E. coli and purified by proprietary chromatographic technique.

Bst DNA Polymerase, Large Fragment

M1213-200 each
EUR 346.8

Bsu DNA Polymerase Large Fragment

M1214-200 each
EUR 477.6

Bst DNA Polymerase Large Fragment

P701-01 800 U
EUR 146.4

Bst DNA Polymerase Large Fragment

P701-02 8000 U
EUR 325.2

Measles Virus Large Polymerase (2059-2183) Protein

20-abx260297
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  • 0.5 mg
  • 100 ug
  • 1 mg

Measles Virus Large Polymerase (2059-2183) (Recombinant)

22060555-1 100 µg
EUR 264.47

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07843 100µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07844 500µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07845 1000µg Ask for price

Bst II DNA Polymerase Large Fragment

P702-01 1,600 U
EUR 27.5

DNA Polymerase I Large (Klenow) Fragment

M1212-100 each
EUR 333.6

DNA Polymerase I, Large (Klenow) Fragment

RK20525 200U
EUR 29

Recombinant (E.Coli) Measles Virus Large Polymerase (2059-2183)

RP-653 100 ug
EUR 343.2

Bst II Pro DNA Polymerase Large Fragment

P703-01 1,600 U
EUR 36.73

Bst II Pro DNA Polymerase Large Fragment

P703-02 8,000 U
EUR 145

Bsu DNA Polymerase Large Fragment - 200 units

3582 200units
EUR 65
Description: Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity (1)Product Includes:Bsu DNA Polymerase I, Large fragment10x Bsu DNA Polymerase I reaction buffer

Sau DNA Polymerase Large Fragment - 200 units

3592 200units
EUR 115
Description: Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.Product Includes:Sau DNA Polymerase I, Large fragment10x Sau DNA Polymerase I reaction buffer

Bst II LAMP DNA Polymerase (Large Fragment) 800U

DP003-0100 100 μl
EUR 15

Recombinant (E.Coli) purified Measles virus Large Polymerase (2059-2183)

RP-1612 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 2059-2183)

VAng-Lsx0399-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 2059-2183), recombinant protein from E. coli.

DNA Polymerase I, Large (Klenow) Fragment - 1,000 units

3262 1/EA
EUR 346.8

Recombinant MeV Polymerase Protein (aa 58-149)

VAng-Lsx0394-inquire inquire Ask for price
Description: MeV Polymerase (aa 58-149), recombinant protein from E. coli.

Cenarchaeum symbiosum DNA polymerase II large subunit (polC)

1-CSB-EP370095DRA
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  • 100ug
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  • 1MG
  • 200ug
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Description: Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial expressed in E.coli

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

CSB-EP370095DRA 3498 mg Ask for price

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial

AP77127 1mg
EUR 3015

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-100ug 100ug
EUR 735.9

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-1mg 1mg
EUR 3078.4

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-20ug 20ug
EUR 519.2

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

Taq Polymerase

PCR-211L 5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-211S 200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-420-10KU 10kilo units
EUR 1043.5

Taq Polymerase

PCR-420-1KU 1kilo unit
EUR 222.3

Taq Polymerase

MBT060-1000U 1 unit
EUR 52.73
Description: Taq Polymerase

Taq Polymerase

MBT060-5000U 1 unit
EUR 204.76
Description: Taq Polymerase

Taq Polymerase

MBT060-500U 1 unit
EUR 35.36
Description: Taq Polymerase

DNA Polymerase

abx461001-1096tests 10 × 96 tests Ask for price

DNA Polymerase

abx461001-596tests 5 × 96 tests Ask for price

DNA Polymerase

abx461001-96tests 96 tests
EUR 425

mtRNA polymerase

E8ER1913-34 100ul
EUR 275
Description: Available in various conjugation types.

HBV polymerase (455 - 463)

5-01273 4 x 5mg Ask for price

(untagged)-Human cDNA FLJ30579 fis, clone BRAWH2006989, weakly similar to DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT (EC 2.7.7.6)

SC312687 10 µg Ask for price

T7 RNA Polymerase (5000U)

9K-005-0004 5000U
EUR 316.82

T7 RNA Polymerase

E041 100 μl (5000 U)
EUR 65
Description: T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.

DNA polymerase mu

E8ER1907-58 100ul
EUR 275
Description: Available in various conjugation types.

T4 DNA Polymerase

M1211-100 each
EUR 385.2

T7 RNA Polymerase

K1083-100000 100000U
EUR 512
Description: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

T7 RNA Polymerase

K1083-25000 25000U
EUR 160
Description: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

T7 RNA Polymerase

K1083-5000 5000U
EUR 40
Description: DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences

T7 RNA Polymerase

PCR-603L 5 x 4000 units
EUR 251.6

T7 RNA Polymerase

PCR-603S 4000units
EUR 63

T4 DNA polymerase

N101-01 2000 U
EUR 223

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

SP6 RNA Polymerase (5000U)

9K-005-0003 5000U
EUR 462.47

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Pfu DNA Polymerase

EQ008 50μl
EUR 68

Pfu DNA Polymerase

enz-265 100U
EUR 145
Description: Recombinant Pfu DNA Polymerase

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

PFU DNA Polymerase

9003-2500 each
EUR 614.4

PFU DNA Polymerase

9003-500 each
EUR 216

Laq? DNA Polymerase

9004-2500 each
EUR 732

Laq? DNA Polymerase

9004-500 each
EUR 261.6

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

SP6 RNA Polymerase

K1096-10000 10000U
EUR 72
Description: SP6 RNA Polymerase, highly specific identification of SP6 promoter sequences

SP6 RNA Polymerase

K1096-2000 2000U
EUR 28
Description: SP6 RNA Polymerase, highly specific identification of SP6 promoter sequences

SP6 RNA Polymerase

K1096-5000 5000U
EUR 40
Description: SP6 RNA Polymerase, highly specific identification of SP6 promoter sequences

Pfu DNA Polymerase

S116 1x250 units
EUR 91.2

Pfu DNA Polymerase

S117 2x250 units
EUR 112.8

Pfu DNA Polymerase

S118 10x250 units
EUR 363.6

Bst DNA Polymerase

S600 2000 U
EUR 128.4

Pfu-X Polymerase

PCR-207L 500units
EUR 362
Description: Pyrococcus furiosus, recombinant, E. coli

Pfu-X Polymerase

PCR-207S 100units
EUR 90.4
Description: Pyrococcus furiosus, recombinant, E. coli

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

DNA Polymerase Mix

abx461002-1096tests 10 × 96 tests Ask for price

DNA Polymerase Mix

abx461002-596tests 5 × 96 tests Ask for price

DNA Polymerase Mix

abx461002-96tests 96 tests
EUR 1262.5

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

DNA Polymerase beta

E8ET1705-12 100ul
EUR 275
Description: Available in various conjugation types.

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Phi29 DNA Polymerase

E014 1000 U (100 μl)
EUR 145
Description: Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates.

DNA polymerase delta

E8ER1907-56 100ul
EUR 275
Description: Available in various conjugation types.

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 104.4

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 189.6

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

Ready? DNA Polymerase

M1146-1000 each
EUR 398.4

Use of Taq DNA polymerase and G-quadruplex 2, 6-diamidoantoanthraquinone bonding BSU-1051, we find that the BSU-1051 leads to improved arrest of the synthesis of the DNA in the presence of K + by stabilizing an intramolecular structure G-quadruplex formed by four repetitions of TTGGGG or TTAGGG in the model pane. The data provides additional evidence that BSU-1051 modules the telomerase activity by stabilizing G-quadruple telomer DNA and point to a polymerase stop test as a sensitive method for screening for interactive agents of G- Quadruplex with potential clinical utility.