CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.

The genetic diversity of a vesicular stomatitis virus population was analyzed by RT-PCR, cloning and sequencing of about 500 nucleotide regions of the virus genome. PCR amplifications were performed in parallel experiments with DNA Taq and PFU polymerases and significant differences were observed. Between 10 and 22 mutations were detected when virus populations were analyzed by TAQ amplification (20 clones of each region), while the amplification of the same samples with PFU revealed between 0 and 5 mutations. PCR loyalty tests, carried out under the same PCR conditions as those used in the population analysis, have shown that the Taq error rate estimation of 0.27 x 10 (-4) Enporations by PB by Cycle was included in the range estimated elsewhere of the amplification of the recombinant PCR plasmids (0.27-0.85 x 10 (-4) Errors by BP per cycle) or functional tests (0.2-2 x 10 ( -4) Errors by PB per cycle).

The TAQ error rate has been found to be 9.3 times higher than the PFU error rate with DNA as a model, and about 10 times higher with the CDNCs obtained by inverse transcription of models. Viral RNA from natural populations. In this study, we discuss (i) the implications of TAQ errors on the analysis of genetic variability, based on both frequency and nature (synonymous replacement) of the substitutions observed and (ii) the size of the Sample required to evaluate genetics variability in a virus population generated by a single infection. In this project, a real-time polymerase chain reaction (PCR) was used to study the mechanism of PCR inhibition by examining the effect of the amplicon length, the melting temperature and of the sequence.

First specially designed with three different amplicon lengths and three different melting temperatures have been used to target a single homozygous allele in the Humert01 locus. The effect on the efficiency of the amplification of each pair of primers has been determined by adding different concentrations of various PCR inhibitors to the reaction mixture. The results show that various inhibition mechanisms can occur during the PCR process based on the type of co-extract inhibitor. These include TAQ inhibition, the binding of DNA templates and the effects on the efficiency of the reaction.

PCR-resistant PCR-resistant Taq DNA mutants allow for amplification of total blood DNA and gross soil samples.

Powerful PCR inhibitors in blood and soil samples can cause false negative results of PCR-based clinical and lawy tests. We show that the effect of these inhibitors is mainly on the TAQ DNA polymerase, because the mutational modification of the polymerase can overcome the inhibition insofar as no purification of the DNA is now required. N-terminal removal (Klentaq1) is an inhibition of 10 to 100 times resistant to total blood relative to the Taq Wild Type TAQ (W.T.) Taq, which is strongly inhibited by 0.1 to 1% blood. Other mutations at Codon 708, both in Klentaq 1 and Taq, give increased resistance to various PCR reaction inhibitors, including total blood, plasma, hemoglobin, lactoferrin, IgG. Serum, soil extracts and humic acid, as well as high concentrations of inserted dyes.

The blood PCR inhibitors can mainly reduce the speed of extension of the W.T DNA Taq polymerase with respect to mutant enzymes. Human genomic targets to a human copy are easily amplified from total blood or raw soil extract, without pretreatment to purify the template DNA, and the permitted increase in the dye concentration overcomes the background of Fluorescence and quenching in real-time blood PCR.

CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.
CONTRIBUTION OF ERRORS INDUCED BY THE TAQ POLYMERASE TO ESTIMATE THE DIVERSITY OF RNA VIRUS.

DNA removal contaminant in the reaction reagents of the polymerase chain: implications for a general approach to the detection of unutaneous pathogens.

The analysis based on comparisons of RNA 16S sequences provides a quick and reliable approach to identifying human pathogens. By leading the primers of oligonucleotides to the sequences preserved throughout the kingdom of ebacterial, the bacterial ribosomal DNA sequences of substantially any member of the kingdom of the ebacteria can be amplified by the reaction of the chain of the polymerase and subsequently analyzed by the determination of the sequence. Indeed, automated wide range amplification systems, sequencing and data analysis are now feasible and can be founded on the next generation of automated microbial identification systems.

However, the identification of the pathogens by this strategy is hampered by the frequent contamination of the reagents used for the amplification reaction, in particular the Taq polymerase, with an exogenous bacterial DNA. Here, we describe detailed surveys of the use of 8-methoxypsorane UV light and the long waves to remove the contaminant DNA in the reaction reagents of the polymerase chain. The clinical utility of the developed procedure has been demonstrated in a case of pacifacillary osteomyelitis for which no specific bacterial agent had been cultivated. We have developed and characterized a test for the interactive compounds of G-quadruplex that use the fact that G-rich DNA models have barriers to the synthesis of DNA by DNA polymerases.

OPPA01109-100UG - MV Large Polymerase 2059-2183 a.a Protein

OPPA01109-100UG 100ug
EUR 278

Recombinant (E.Coli) Measles Virus Large Polymerase (58-149)

RP-651 100 ug
EUR 343.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-1mg

QP12758-1mg 1mg
EUR 1513.2

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-100ug

QP12758-100ug 100ug
EUR 261.6

Recombinant Measles Virus MV Large Polymerase 58-149 a. a Protein, Untagged, E.coli-500ug

QP12758-500ug 500ug
EUR 795.6

Recombinant (E.Coli) purified Measles virus Large Polymerase (58-149)

RP-1613 100 ug
EUR 416.4

Recombinant MeV Active Large Polymerase Protein (aa 58-149)

VAng-Lsx0397-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 58-149), recombinant protein from E. coli.

Measles Virus, Large RNA Polymerase, aa58-149, Recombinant

MBS636659-01mg 0.1mg
EUR 475

Measles Virus, Large RNA Polymerase, aa58-149, Recombinant

MBS636659-05mg 0.5mg
EUR 925

Measles Virus, Large RNA Polymerase, aa58-149, Recombinant

MBS636659-5x05mg 5x0.5mg
EUR 4015

Recombinant MeV Large Polymerase Protein

VAng-Lsx0385-inquire inquire Ask for price
Description: Recombinant MeV Large Polymerase containing the large polymerase immunodominant regions, 58-149 amino acids was expressed in E. coli and purified by proprietary chromatographic technique.

Recombinant MeV Polymerase Protein (aa 58-149)

VAng-Lsx0394-inquire inquire Ask for price
Description: MeV Polymerase (aa 58-149), recombinant protein from E. coli.

Bsu DNA Polymerase Large Fragment

3587 2000Units(5units/µl)
EUR 316

Bst DNA Polymerase, Large Fragment

M1213-200 each
EUR 346.8

Bsu DNA Polymerase Large Fragment

M1214-200 each
EUR 477.6

Bst DNA Polymerase Large Fragment

P701-01 800 U
EUR 146.4

Bst DNA Polymerase Large Fragment

P701-02 8000 U
EUR 325.2

Bsu DNA Polymerase Large Fragment

MBS516634-1000Units 1000Units
EUR 370

Bsu DNA Polymerase Large Fragment

MBS516634-200Units 200Units
EUR 225

Bsu DNA Polymerase Large Fragment

MBS516634-5x1000Units 5x1000Units
EUR 1310

Sau DNA Polymerase Large Fragment

MBS517612-1000Units 1000Units
EUR 570

Sau DNA Polymerase Large Fragment

MBS517612-200Units 200Units
EUR 455

Sau DNA Polymerase Large Fragment

MBS517612-5x1000Units 5x1000Units
EUR 2310

Measles Virus Large Polymerase (2059-2183) Protein

20-abx260297
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  • 0.5 mg
  • 100 ug
  • 1 mg

Measles Virus Large Polymerase (2059-2183) (Recombinant)

22060555-1 100 µg
EUR 264.47

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07843 100µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07844 500µg Ask for price

Recombinant Measles Virus Large Polymerase (2059-2183)

7-07845 1000µg Ask for price

Bst II DNA Polymerase Large Fragment

P702-01 1,600 U
EUR 27.5

Bst II DNA Polymerase Large Fragment

P702-01-1600U 1,600 U
EUR 28.48

DNA Polymerase I Large (Klenow) Fragment

M1212-100 each
EUR 333.6

DNA Polymerase I, Large (Klenow) Fragment

RK20525 200U
EUR 29

DNA Polymerase I, Large (Klenow) Fragment

MBS9141653-200Units 200Units
EUR 145

Recombinant (E.Coli) Measles Virus Large Polymerase (2059-2183)

RP-653 100 ug
EUR 343.2

Bst II Pro DNA Polymerase Large Fragment

P703-01 1,600 U
EUR 36.73

Bst II Pro DNA Polymerase Large Fragment

P703-02 8,000 U
EUR 145

Bst II Pro DNA Polymerase Large Fragment

P703-01-1600U 1,600 U
EUR 38.04

Bst II Pro DNA Polymerase Large Fragment

P703-02-8000U 8,000 U
EUR 150.15

Bsu DNA Polymerase Large Fragment - 200 units

3582 200units
EUR 65
Description: Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity (1)Product Includes:Bsu DNA Polymerase I, Large fragment10x Bsu DNA Polymerase I reaction buffer

Sau DNA Polymerase Large Fragment - 200 units

3592 200units
EUR 115
Description: Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.Product Includes:Sau DNA Polymerase I, Large fragment10x Sau DNA Polymerase I reaction buffer

Measles Virus, Large Polymerase, aa2059-2183, Recombinant

MBS635727-01mg 0.1mg
EUR 475

Measles Virus, Large Polymerase, aa2059-2183, Recombinant

MBS635727-05mg 0.5mg
EUR 925

Measles Virus, Large Polymerase, aa2059-2183, Recombinant

MBS635727-5x05mg 5x0.5mg
EUR 4015

Glycerol Free Bsu DNA Polymerase Large Fragment

3582GF 200Units(5units/µl)
EUR 124

Glycerol Free Bsu DNA Polymerase Large Fragment

3585GF 1000Units(5units/µl
EUR 360

Recombinant (E.Coli) purified Measles virus Large Polymerase (2059-2183)

RP-1612 100 ug
EUR 416.4

Bst II LAMP DNA Polymerase (Large Fragment) 800U

DP003-0100 100 μl
EUR 15

Recombinant MeV Active Large Polymerase Protein (aa 2059-2183)

VAng-Lsx0399-inquire inquire Ask for price
Description: Measles Active Large Polymerase protein (aa 2059-2183), recombinant protein from E. coli.

DNA Polymerase I, Large (Klenow) Fragment - 1,000 units

3262 1/EA
EUR 346.8

Cenarchaeum symbiosum DNA polymerase II large subunit (polC)

1-CSB-EP370095DRA
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial expressed in E.coli

Bst II Pro DNA Polymerase Large Fragment (Glycerol-free)

PL703-01-1600U 1,600 U
EUR 39.79

Bst II Pro DNA Polymerase Large Fragment (Glycerol-free)

PL703-02-8000U 8,000 U
EUR 158.05

Recombinant Pyrococcus abyssi DNA polymerase II large subunit (polC)

MBS1334349-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1790

Recombinant Pyrococcus abyssi DNA polymerase II large subunit (polC)

MBS1334349-002mgEColi 0.02mg(E-Coli)
EUR 1575

Recombinant Pyrococcus abyssi DNA polymerase II large subunit (polC)

MBS1334349-002mgYeast 0.02mg(Yeast)
EUR 1550

Recombinant Pyrococcus abyssi DNA polymerase II large subunit (polC)

MBS1334349-01mgEColi 0.1mg(E-Coli)
EUR 1900

Recombinant Pyrococcus abyssi DNA polymerase II large subunit (polC)

MBS1334349-01mgYeast 0.1mg(Yeast)
EUR 1820

Recombinant Pyrococcus kodakaraensis DNA polymerase II large subunit (polC)

MBS1335655-INQUIRE INQUIRE Ask for price

Recombinant Pyrococcus furiosus DNA polymerase II large subunit (polC), partial

MBS1244546-INQUIRE INQUIRE Ask for price

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit(polC),partial

AP77127 1mg
EUR 3015

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

CSB-EP370095DRA 3498 mg Ask for price

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

MBS1140153-002mgEColi 0.02mg(E-Coli)
EUR 450

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

MBS1140153-01mgEColi 0.1mg(E-Coli)
EUR 650

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

MBS1140153-1mgEColi 1mg(E-Coli)
EUR 2050

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC), partial

MBS1140153-5x1mgEColi 5x1mg(E-Coli)
EUR 8960

Recombinant Pyrococcus horikoshii DNA polymerase II large subunit (polC), partial

MBS1112848-INQUIRE INQUIRE Ask for price

Recombinant Haloquadratum walsbyi DNA polymerase II large subunit (polC), partial

MBS1300996-INQUIRE INQUIRE Ask for price

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-100ug 100ug
EUR 735.9

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-1mg 1mg
EUR 3078.4

Recombinant Cenarchaeum symbiosum DNA polymerase II large subunit (polC) ,partial

RPC22828-20ug 20ug
EUR 519.2

Recombinant Picrophilus torridus DNA polymerase II large subunit (polC), partial

MBS1405379-INQUIRE INQUIRE Ask for price

Recombinant Nanoarchaeum equitans DNA polymerase II large subunit (polC), partial

MBS1387628-INQUIRE INQUIRE Ask for price

Recombinant Methanopyrus kandleri DNA polymerase II large subunit (polC), partial

MBS1381161-INQUIRE INQUIRE Ask for price

Recombinant Methanosarcina mazei DNA polymerase II large subunit (polC), partial

MBS1332694-INQUIRE INQUIRE Ask for price

Recombinant Archaeoglobus fulgidus DNA polymerase II large subunit (polC), partial

MBS1194039-INQUIRE INQUIRE Ask for price

Recombinant Methanococcus vannielii DNA polymerase II large subunit (polC), partial

MBS1129442-INQUIRE INQUIRE Ask for price

Recombinant Haloarcula marismortui DNA polymerase II large subunit (polC), partial

MBS1296146-INQUIRE INQUIRE Ask for price

Recombinant Thermoplasma volcanium DNA polymerase II large subunit (polC), partial

MBS1302599-INQUIRE INQUIRE Ask for price

Recombinant Natronomonas pharaonis DNA polymerase II large subunit (polC), partial

MBS1465387-INQUIRE INQUIRE Ask for price

Recombinant Halobacterium salinarum DNA polymerase II large subunit (polC), partial

MBS1472754-INQUIRE INQUIRE Ask for price

Recombinant Methanosarcina barkeri DNA polymerase II large subunit (polC), partial

MBS1328736-INQUIRE INQUIRE Ask for price

Recombinant Methanosaeta thermophila DNA polymerase II large subunit (polC), partial

MBS1178109-INQUIRE INQUIRE Ask for price

Recombinant Methanoculleus marisnigri DNA polymerase II large subunit (polC), partial

MBS1042553-INQUIRE INQUIRE Ask for price

Recombinant Methanococcus maripaludis DNA polymerase II large subunit (polC), partial

MBS1054422-INQUIRE INQUIRE Ask for price

Recombinant Methanococcus maripaludis DNA polymerase II large subunit (polC), partial

MBS1111712-INQUIRE INQUIRE Ask for price

Recombinant Methanococcus maripaludis DNA polymerase II large subunit (polC), partial

MBS1229731-INQUIRE INQUIRE Ask for price

Recombinant Methanococcus maripaludis DNA polymerase II large subunit (polC), partial

MBS1314070-INQUIRE INQUIRE Ask for price

Recombinant Methanosphaera stadtmanae DNA polymerase II large subunit (polC), partial

MBS1280951-INQUIRE INQUIRE Ask for price

Recombinant Thermoplasma acidophilum DNA polymerase II large subunit (polC), partial

MBS1324459-INQUIRE INQUIRE Ask for price

Recombinant Methanococcoides burtonii DNA polymerase II large subunit (polC), partial

MBS1286304-INQUIRE INQUIRE Ask for price

Recombinant Methanospirillum hungatei DNA polymerase II large subunit (polC), partial

MBS1400111-INQUIRE INQUIRE Ask for price

Recombinant Methanosarcina acetivorans DNA polymerase II large subunit (polC), partial

MBS1384903-INQUIRE INQUIRE Ask for price

Recombinant Methanocorpusculum labreanum DNA polymerase II large subunit (polC), partial

MBS1148683-INQUIRE INQUIRE Ask for price

Recombinant Methanocaldococcus jannaschii DNA polymerase II large subunit (polC), partial

MBS1412704-INQUIRE INQUIRE Ask for price

Recombinant Methanothermobacter thermautotrophicus DNA polymerase II large subunit (polC), partial

MBS1095402-INQUIRE INQUIRE Ask for price

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit)

MBS6001186-02mL 0.2(mL
EUR 695

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit)

MBS6001186-5x02mL 5x0.2mL
EUR 2975

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (AP)

MBS6335653-02mL 0.2mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (AP)

MBS6335653-5x02mL 5x0.2mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (APC)

MBS6335654-02mL 0.2mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (APC)

MBS6335654-5x02mL 5x0.2mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (PE)

MBS6335663-02mL 0.2mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (PE)

MBS6335663-5x02mL 5x0.2mL
EUR 4250

Recombinant Uncultured methanogenic archaeon RC-I DNA polymerase II large subunit (polC), partial

MBS1418428-INQUIRE INQUIRE Ask for price

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (Biotin)

MBS6335655-02mL 0.2mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (Biotin)

MBS6335655-5x02mL 5x0.2mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (FITC)

MBS6335656-02mL 0.2mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (FITC)

MBS6335656-5x02mL 5x0.2mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 405)

MBS6335658-01mL 0.1mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 405)

MBS6335658-5x01mL 5x0.1mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 490)

MBS6335659-01mL 0.1mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 490)

MBS6335659-5x01mL 5x0.1mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 550)

MBS6335660-01mL 0.1mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 550)

MBS6335660-5x01mL 5x0.1mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 650)

MBS6335661-01mL 0.1mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 650)

MBS6335661-5x01mL 5x0.1mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 750)

MBS6335662-01mL 0.1mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (MaxLight 750)

MBS6335662-5x01mL 5x0.1mL
EUR 4250

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (Azide free) (HRP)

MBS6335657-02mL 0.2mL
EUR 980

POLR1A, CT (POLR1A, DNA-directed RNA polymerase I subunit RPA1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194kD subunit) (Azide free) (HRP)

MBS6335657-5x02mL 5x0.2mL
EUR 4250

POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) APC

MBS6138349-01mL 0.1(mL
EUR 875

POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) APC

MBS6138349-5x01mL 5x0.1mL
EUR 3800

Use of Taq DNA polymerase and G-quadruplex 2, 6-diamidoantoanthraquinone bonding BSU-1051, we find that the BSU-1051 leads to improved arrest of the synthesis of the DNA in the presence of K + by stabilizing an intramolecular structure G-quadruplex formed by four repetitions of TTGGGG or TTAGGG in the model pane. The data provides additional evidence that BSU-1051 modules the telomerase activity by stabilizing G-quadruple telomer DNA and point to a polymerase stop test as a sensitive method for screening for interactive agents of G- Quadruplex with potential clinical utility.