The thermal stability of Taq DNA polymerase known, and is the basis for use in PCR. A comparison of the thermodynamic characterization of a large fragment of Taq domain (Klentaq) and E. coli (Klenow) of DNA polymerase has done by getting the Gibbs-Helmholtz stability curve full folding free energy (AG) versus temperature. This analysis provides temperature dependencies folding enthalpy and entropy (ΔH and ΔS), and heat capacity (ΔCp) fold.

If increased or enhanced non-covalent bonding in the original state is responsible for increasing the thermal stabilization of proteins, such as frequently asked questions, then folding enthalpy enhanced benefit should, in general, observed for thermophilic proteins. However, for Klenow-Klentaq homologous pairs, folding enthalpy (ΔHfold) of Klentaq far less profitable than Klenow at all temperatures. Instead, it was found that the free energy of folding extreme Klentaq (ΔGfold) comes from the entropic penalty significantly reduces the fold (ΔSfold). In addition, the heat capacity change at a similar fold for Klenow and Klentaq.

Along with this new data, the extended analysis of comparable thermodynamic data is available for 17 pairs of mesophilic-thermophilic proteins other (where applicable thermodynamics enough data available) show the same pattern in seven out of 18 total system. When analyzed with this approach, which is better known as “reduced ΔCp mechanism” for protein thermal stabilization (observed in six different 18 systems) often manifests as a shift from stabilization temperature dependent enthalpy driven model entropic-penalty reduction.

Taq DNA polymerase mutant with improved ability elongation as reagents useful for genetic engineering.

DNA polymerase is widely used for manipulating DNA in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. thermostable DNA polymerase is very useful and can be quite worthwhile after the development of PCR technology. A DNA polymerases from Thermus aquaticus (Taq polymerase) is the most well-known DNA polymerase as PCR enzyme, and has been widely used around the world.

In this study, the gene fragment of family A DNA polymerase was amplified by PCR from the DNA of microorganisms in environmental soil samples, using a set of primers for two conserved regions. The corresponding region of the pol gene for Taq polymerase gene fragment was replaced with reinforced, and a variety of chimeric DNA polymerases prepared. Based on these properties chimeric enzymes and their sequence, two residues, E742 and A743, in the Taq polymerase was found to be important for the ability of elongation.

Taq polymerase with mutations in the 742 and 743 actually showed a higher affinity DNA and primer extension capabilities faster. These factors also affect the performance of PCR on DNA polymerase, and the PCR yield improvement was observed with Taq polymerase mutants.
coumarin derivatives were prepared using a natural product isolated from a plant belonging to the genus Pterocaulon (Asteraceae) and commercial drugs.

Taq DNA Polymerase
9001-2500	Biovision	each	EUR 614.4

Taq DNA Polymerase
9001-500	Biovision	each	EUR 229.2

Taq DNA polymerase
BT10101	Bioatlas	500U	EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10102	Bioatlas	1000U	EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase
BT10103	Bioatlas	2000U	EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00103	Cusabio	500U	EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00104	Bioatlas	1000U	EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
BA00105	Bioatlas	2500U	EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase
L7051001	Biochain	1000 unit	EUR 159

Taq DNA Polymerase
L7051200	Biochain	200 unit	EUR 41

Taq DNA Polymerase (3000U)
9K-001-0002	Bio Basic	3000U	EUR 582

Taq DNA Polymerase (6000U)
9K-001-0033	Bio Basic	6000U	EUR 1234.5

Taq DNA Polymerase (1000U)
9K-001-0035	Bio Basic	1000U	EUR 418.62

Taq DNA Polymerase (500U)
9K-001-0001	Bio Basic	500U	EUR 209.29

Taq HS DNA Polymerase
P132-d1	Vazyme	500 U	EUR 38.28

Taq HS DNA Polymerase
P132-d2	Vazyme	1,000 U	EUR 71.34

Taq HS DNA Polymerase
P132-d3	Vazyme	5,000 U	EUR 324.8

Taq DNA Polymerase (PCR)
G008	ABM	5000 U (1.0 ml)	EUR 246

Taq DNA Polymerase (PCR)
G009	ABM	1000 U (200 ul)	EUR 104.4

Taq DNA Polymerase (PCR)
G126	ABM	10000 U (2 x 1.0 ml)	EUR 370.8

Taq DNA polymerase (+dNTPs)
02-001	Sceti	200U	EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)
02-001-5	Sceti	5×200U	EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)
02-011	Sceti	200U	EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)
02-011-5	Sceti	5×200U	EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase
S101	GeneOn	500 units	EUR 55.2

Maximo Taq DNA Polymerase
S102	GeneOn	5x500 units	EUR 128.4

Maximo Taq DNA Polymerase
S103	GeneOn	20x500 units	EUR 393.6

Taq DNA Polymerase, 5units/㎕
T2200-050	GenDepot	500units	EUR 157.2

Taq DNA Polymerase, 5units/㎕
T2200-100	GenDepot	2x500 units	EUR 230.4

Taq DNA Polymerase, 5units/㎕
T2200-200	GenDepot	4x500 units	EUR 375.6

Hotstart Taq DNA Polymerase
L7052001	Biochain	1000 units	EUR 567

Hotstart Taq DNA Polymerase
L7052250	Biochain	250 units	EUR 159

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-025	GenDepot	250 unit	EUR 200.4

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-050	GenDepot	2X250 unit	EUR 300

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-100	GenDepot	4X250 Unit	EUR 505.2

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2
P0310-250	GenDepot	10X250 Unit	EUR 886.8

Champagne Taq DNA Polymerase
P122-d1	Vazyme	500 U (2.5 U/μl)	EUR 212.4

Champagne Taq DNA Polymerase
P122-d2	Vazyme	500 U (5 U/μl)	EUR 212.4

Champagne Taq DNA Polymerase
P122-d3	Vazyme	500 U (10 U/μl)	EUR 212.4

amfiXpand PCR Master Mix
P0331-010	GenDepot	2X50 rxns	EUR 181.2

amfiXpand PCR Master Mix
P0331-025	GenDepot	5X50 rxns	EUR 327.6

amfiXpand PCR Master Mix
P0331-050	GenDepot	10X50 rxns	EUR 537.6

amfiXpand PCR Master Mix
P0331-250	GenDepot	50x50 rxns	EUR 2247.6

ACTaq? Taq DNA Polymerase, 1000U
E2100-1000U	ACTGene	each	EUR 260.4

Taq DNA Polymerase - 5,000 units
3245	Intact Genomics	1/EA	EUR 620.4

Recombinant Taq DNA Polymerase
7-03634	CHI Scientific	1,000U	Ask for price

Recombinant Taq DNA Polymerase
7-03635	CHI Scientific	3,000U	Ask for price

Recombinant Taq DNA Polymerase
7-03636	CHI Scientific	10,000U	Ask for price

Recombinant Taq Dna Polymerase
27079804	Scientific Laboratory Supplies	EACH	EUR 117.6

Recombinant Taq Dna Polymerase
27079806	Scientific Laboratory Supplies	EACH	EUR 639.6

Taq DNA Polymerase (Recombinant)
abx073521-10000U	Abbexa	10000 U	EUR 710.4

Taq DNA Polymerase (Recombinant)
abx073521-1000U	Abbexa	1000 U	EUR 276

Taq DNA Polymerase (Recombinant)
abx073521-3000U	Abbexa	3000 U	EUR 393.6

ACTaq? Taq DNA Polymerase, 250U
E2100-250U	ACTGene	each	EUR 122.4

Taq Plus DNA Polymerase
P201-01	Vazyme	250 U	EUR 147.6

Taq Plus DNA Polymerase
P201-02	Vazyme	1000 U	EUR 213.6

Taq Plus DNA Polymerase
P201-03	Vazyme	3000 U	EUR 382.8

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-050	GenDepot	500 units	EUR 198

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-102	GenDepot	25x500 units	EUR 1712.4

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2
P0701-500	GenDepot	10X500 units	EUR 886.8

Taq DNA Polymerase, 5u/ul
B0089(D0089)	Bio Basic	5X200U	EUR 112.2

amfiXpand 2x Reaction Buffer
P0333-001	GenDepot	1ml	EUR 121.2

Genorise® Taq DNA Polymerase
GR108007	Genorise Scientific	200 tests	EUR 230

YEA taq DNA Polymerase wiodNTP
FYT011-500U	Yeastern Biotech	500U	Ask for price

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-500	GenDepot	500 units	EUR 262.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-502	GenDepot	2x500 units	EUR 424.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-510	GenDepot	10x500 units	EUR 1647.6

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-525	GenDepot	25x500 units	EUR 3624

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included
P7002-550	GenDepot	50x500 units	EUR 6459.6

Taq Plus DNA Polymerase (PCR)
G012	ABM	250 U (50 ul)	EUR 87.6

Taq Plus DNA Polymerase (PCR)
G040	ABM	1000 U (200 ul)	EUR 127.2

Recombinant (E.Coli) Taq DNA Polymerase
RP-394	Alpha Diagnostics	1000 IU	EUR 196.8

Taq DNA Polymerase Polyclonal Antibody
7018-100	Biovision	each	EUR 424.8

Taq DNA Polymerase Polyclonal Antibody
7018-30T	Biovision	each	EUR 175.2

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ001	Allele Biotech	1000U	Ask for price

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ100	Allele Biotech	100U	Ask for price

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200	Allele Biotech	200U	Ask for price

Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500	Allele Biotech	500U	Ask for price

YEA taq DNA Polymerase wi dNTP
FYT001-500U	Yeastern Biotech	500U	Ask for price

ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U	ACTGene	each	EUR 272.4

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-01	Vazyme	1000 U	EUR 145.2

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02	Vazyme	5000 U	EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03	Vazyme	10000 U	EUR 314.4

Taq DNA Polymerase (Mg2+ free Buffer)
P102-01	Vazyme	1000 U	EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)
P102-02	Vazyme	5000 U	EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)
P102-03	Vazyme	10000 U	EUR 314.4

ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U	ACTGene	each	EUR 126

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U	Alpha Diagnostics	Custom	Ask for price

Cheetah Hot Start Taq DNA Polymerase
29050	Biotium	500EU	EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

Taq Plus DNA Polymerase, 5u/ul
D0090	Bio Basic	200U	EUR 94.8

Recombinant Taq Plus DNA Polymerase
7-03631	CHI Scientific	250U	Ask for price

Recombinant Taq Plus DNA Polymerase
7-03632	CHI Scientific	750U	Ask for price

Recombinant Taq Plus DNA Polymerase
7-03633	CHI Scientific	2,500U	Ask for price

Taq Plus DNA Polymerase (Recombinant)
abx073522-2500U	Abbexa	2.500 U	EUR 710.4

Taq Plus DNA Polymerase (Recombinant)
abx073522-250U	Abbexa	250 U	EUR 276

Taq Plus DNA Polymerase (Recombinant)
abx073522-750U	Abbexa	750 U	EUR 393.6

Taq HS DNA Polymerase (Glycerol-free)
QL101-01	Vazyme	1,000 U	EUR 71.2

Taq HS DNA Polymerase (Glycerol-free)
QL101-02	Vazyme	5,000 U	EUR 324.8

Taq HS DNA Polymerase (Glycerol-free)
QL101-03	Vazyme	50,000 U	EUR 2900

Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B01	GenDepot	10,000 units	EUR 1388.4

Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B05	GenDepot	5x10,000 units	EUR 6540

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B	Alpha Diagnostics	Custom	Ask for price

Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018	Bio Basic	6000U	EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031	Bio Basic	500U	EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032	Bio Basic	3000U	EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034	Bio Basic	1000U	EUR 518.84

3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81	Bio Basic	5x500U	EUR 896.24

Taq DNA Polymerase (High Purity), 5u/ul
HTD0078	Bio Basic	5X200U	EUR 129.6

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-050	GenDepot	500units	EUR 157.2

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-100	GenDepot	2x500 units	EUR 230.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕
T2201-200	GenDepot	4x500 units	EUR 375.6

Genorise® CLT Taq DNA Polymerase (1000 U)
GR108003	Genorise Scientific	1000 U	EUR 199

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N140	GeneOn	500 units	EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N142	GeneOn	5x500 units	EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free
N144	GeneOn	20x500 units	EUR 440.4

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled
AD-146-F	Alpha Diagnostics	Custom	Ask for price

Taq DNA Polymerase 2x Premix - 500 Reactions
3249	Intact Genomics	1/EA	EUR 228

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)
RK20601	Abclonal	25 U	Ask for price

Recombinant (E.Coli) Taq Plus DNA Polymerase
RP-395	Alpha Diagnostics	250 U	EUR 196.8

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-001	GenDepot	1000 units	EUR 198

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-005	GenDepot	5000 units	EUR 360

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-010	GenDepot	10,000 units	EUR 699.6

Taq DNA Polymerase, Ultra Pure, 5 units/ul
T2016-100	GenDepot	100,000 units	EUR 3462

Fast-Taq DNA Polymerase (500U)
9K-001-0036	Bio Basic	500U	EUR 420.65

amfiXpand Hot-Start Version , Including dNTP&MgCl2
P0334-020	GenDepot	200 units	EUR 313.2

amfiXpand Hot-Start Version , Including dNTP&MgCl2
P0334-040	GenDepot	2X200 units	EUR 498

amfiXpand Hot-Start Version , Including dNTP&MgCl2
P0334-120	GenDepot	6x200 units	EUR 1178.4

amfiXpand Hot-Start Version , Including dNTP&MgCl2
P0334-400	GenDepot	20x200 units	EUR 2895.6

amfiXpand Hot-Start Version , Including dNTP&MgCl2
P0334-800	GenDepot	40x200 units	EUR 5406

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1	Vazyme	1000 U	EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2	Vazyme	5000 U	EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3	Vazyme	10000 U	EUR 490.8

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1	Vazyme	1000 U	EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2	Vazyme	5000 U	EUR 320.4

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3	Vazyme	10000 U	EUR 490.8

Reversible 1-min Hot Start Taq DNA Polymerase
P950	101Bio	-	Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase
P950L	101Bio	-	Ask for price

Taq DNA Polymerase - 5,000 units with separate dNTP Mix
3245d	Intact Genomics	1/EA	EUR 802.8

amfiSure CloneEasy DNA Polymerase
P0325-010	GenDepot	100 units	EUR 278.4

amfiSure CloneEasy DNA Polymerase
P0325-025	GenDepot	250 units	EUR 481.2

Anti-Taq DNA Polymerase Antibody
48369-100ul	SAB	100ul	EUR 399.6

Anti-Taq DNA Polymerase Antibody
48369-50ul	SAB	50ul	EUR 286.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150	GeneOn	500 units	EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152	GeneOn	5x500 units	EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154	GeneOn	20x500 units	EUR 1830

Taq Pro Multiplex DNA Polymerase (High sensitivity)
PM201-01	Vazyme	200 rxns	EUR 72.5

Taq Pro Multiplex DNA Polymerase (High sensitivity)
PM201-02	Vazyme	1,000 rxns	EUR 290

Taq Pro Multiplex DNA Polymerase (High specificity)
PM202-01	Vazyme	200 rxns	EUR 72.5

Taq Pro Multiplex DNA Polymerase (High specificity)
PM202-02	Vazyme	1,000 rxns	EUR 290

amfiSure GC-Rich DNA Polymerase
P0324-010	GenDepot	100 units	EUR 310.8

amfiSure GC-Rich DNA Polymerase
P0324-025	GenDepot	250 units	EUR 505.2

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B01	GenDepot	10,000 units	EUR 1388.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing
T2201-B05	GenDepot	5x10,000 units	EUR 6540

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix
3293d	Intact Genomics	1/EA	EUR 428.4

amfiFusion High Fidelity DNA Polymerase
P0341-010	GenDepot	100 units	EUR 241.2

amfiFusion High Fidelity DNA Polymerase
P0341-020	GenDepot	2x100 units	EUR 360

amfiFusion High Fidelity DNA Polymerase
P0341-050	GenDepot	5X100 units	EUR 772.8

amfiFusion High Fidelity DNA Polymerase
P0341-100	GenDepot	10x100 units	EUR 1340.4

amfiFusion High Fidelity DNA Polymerase
P0341-200	GenDepot	20x100 units	EUR 2344.8

amfiFusion High Fidelity DNA Polymerase
P0341-500	GenDepot	50x100 units	EUR 5470.8

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S113	GeneOn	2x100 rcs	EUR 79.2

Maximo Taq DNA Polymerase (2X pre-mix, ready-to-use)
S114	GeneOn	10x100 rcs	EUR 231.6

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S400	GeneOn	200 units	EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S410	GeneOn	1000 units	EUR 231.6

amfiSure HiFi High Fidelity DNA Polymerase
P0323-010	GenDepot	100 units	EUR 273.6

amfiSure HiFi High Fidelity DNA Polymerase
P0323-025	GenDepot	250 units	EUR 505.2

amfiSure HiFi High Fidelity DNA Polymerase
P0323-050	GenDepot	500 units	EUR 902.4

Anti-Taq DNA Polymerase Conjugated Antibody
C48369	SAB	100ul	EUR 476.4

EZ Taq Polymerase (HotStart)
9K-005-0020	Bio Basic	500U	EUR 245.83

Several molecules have been shown interesting activity against murine myeloid leukemia virus reverse transcriptase (MMLV-RT) (compounds 20 and 28 were produced inhibition with IC50 values ​​of 38.62 and 50.98 pM, respectively) and Taq DNA polymerase (analog 13 and 14 are produced inhibition with IC50 values ​​of 48.08 and 57.88 pM, respectively). The inhibitor may have an interest as antiretroviral chemotherapeutic agents and also in the development of anticancer drugs.