The stability of Taq DNA polymerase results from a reduced entropic folding penalty; identification of other thermophilic proteins with similar folding thermodynamics.

The thermal stability of Taq DNA polymerase known, and is the basis for use in PCR. A comparison of the thermodynamic characterization of a large fragment of Taq domain (Klentaq) and E. coli (Klenow) of DNA polymerase has done by getting the Gibbs-Helmholtz stability curve full folding free energy (AG) versus temperature. This analysis provides temperature dependencies folding enthalpy and entropy (ΔH and ΔS), and heat capacity (ΔCp) fold.

If increased or enhanced non-covalent bonding in the original state is responsible for increasing the thermal stabilization of proteins, such as frequently asked questions, then folding enthalpy enhanced benefit should, in general, observed for thermophilic proteins. However, for Klenow-Klentaq homologous pairs, folding enthalpy (ΔHfold) of Klentaq far less profitable than Klenow at all temperatures. Instead, it was found that the free energy of folding extreme Klentaq (ΔGfold) comes from the entropic penalty significantly reduces the fold (ΔSfold). In addition, the heat capacity change at a similar fold for Klenow and Klentaq.

Along with this new data, the extended analysis of comparable thermodynamic data is available for 17 pairs of mesophilic-thermophilic proteins other (where applicable thermodynamics enough data available) show the same pattern in seven out of 18 total system. When analyzed with this approach, which is better known as “reduced ΔCp mechanism” for protein thermal stabilization (observed in six different 18 systems) often manifests as a shift from stabilization temperature dependent enthalpy driven model entropic-penalty reduction.

The stability of Taq DNA polymerase results from a reduced entropic folding penalty; identification of other thermophilic proteins with similar folding thermodynamics.
The stability of Taq DNA polymerase results from a reduced entropic folding penalty; identification of other thermophilic proteins with similar folding thermodynamics.

Taq DNA polymerase mutant with improved ability elongation as reagents useful for genetic engineering.

DNA polymerase is widely used for manipulating DNA in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. thermostable DNA polymerase is very useful and can be quite worthwhile after the development of PCR technology. A DNA polymerases from Thermus aquaticus (Taq polymerase) is the most well-known DNA polymerase as PCR enzyme, and has been widely used around the world.

In this study, the gene fragment of family A DNA polymerase was amplified by PCR from the DNA of microorganisms in environmental soil samples, using a set of primers for two conserved regions. The corresponding region of the pol gene for Taq polymerase gene fragment was replaced with reinforced, and a variety of chimeric DNA polymerases prepared. Based on these properties chimeric enzymes and their sequence, two residues, E742 and A743, in the Taq polymerase was found to be important for the ability of elongation.

Taq polymerase with mutations in the 742 and 743 actually showed a higher affinity DNA and primer extension capabilities faster. These factors also affect the performance of PCR on DNA polymerase, and the PCR yield improvement was observed with Taq polymerase mutants.
coumarin derivatives
were prepared using a natural product isolated from a plant belonging to the genus Pterocaulon (Asteraceae) and commercial drugs.

amfiXpand PCR Master Mix

P0331-010 2X50 rxns
EUR 181.2

amfiXpand PCR Master Mix

P0331-025 5X50 rxns
EUR 327.6

amfiXpand PCR Master Mix

P0331-050 10X50 rxns
EUR 537.6

amfiXpand PCR Master Mix

P0331-250 50x50 rxns
EUR 2247.6

amfiXpand 2x Reaction Buffer

P0333-001 1ml
EUR 121.2

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

L7051001 1000 unit
EUR 159

Taq DNA Polymerase

L7051200 200 unit
EUR 41

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-025 250 unit
EUR 200.4

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-050 2X250 unit
EUR 300

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-100 4X250 Unit
EUR 505.2

amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

P0310-250 10X250 Unit
EUR 886.8

TM Taq DNA Polymerase

TBS4010 1000 U
EUR 60

Taq HS DNA Polymerase

P132-d1 500 U
EUR 38.28

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 71.34

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 324.8

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 104.4

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-050 500 units
EUR 198

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-102 25x500 units
EUR 1712.4

amfiEco Taq DNA Polymerase, 1 unit/ul,Including dNTP&MgCl2

P0701-500 10X500 units
EUR 886.8

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 567

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 159

Taq DNA Polymerase -1000 units

3243 1000units
EUR 115
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

Recombinant Taq Dna Polymerase

27079804 EACH
EUR 117.6

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 639.6

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase (Recombinant)

22060707-1 1,000 Unit (s)
EUR 137.27

Taq DNA Polymerase (Recombinant)

22060707-2 1500 Unit (s)
EUR 182.1

Taq DNA Polymerase (Recombinant)

22060707-3 5000 Unit (s)
EUR 465.77

Taq DNA Polymerase (Recombinant)

22060707-4 10 KU
EUR 840.96

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

Taq DNA Polymerase (5U/uL)

A1003-500U 500U
EUR 26.4
Description: Biotechnology

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-500 500 units
EUR 262.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-502 2x500 units
EUR 424.8

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-510 10x500 units
EUR 1647.6

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-525 25x500 units
EUR 3624

amfiSure ONE Taq DNA Polymerase, 1unit/ul, MgCl2 and dNTP Included

P7002-550 50x500 units
EUR 6459.6

Taq DNA Polymerase (5U/µl), 2000U

PL1204 each Ask for price

Hot start Taq DNA Polymerase

EQ006 100μl/500U
EUR 200

Taq DNA Polymerase (5U/µl), 500U

PL1202 each Ask for price

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

ACTaq™ Taq DNA Polymerase, 1000U

E2100 1000U
EUR 144.57

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-020 200 units
EUR 313.2

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-040 2X200 units
EUR 498

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-120 6x200 units
EUR 1178.4

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-400 20x200 units
EUR 2895.6

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-800 40x200 units
EUR 5406

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Taq Plus DNA Polymerase (PCR)

G012 250 U (50 ul)
EUR 87.6

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
EUR 127.2

Taq DNA Polymerase Polyclonal Antibody

7018-100 each
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T each
EUR 175.2

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

HotStart Taq DNA Polymerase (5U/uL)

A4144-250U 250U
EUR 66
Description: Biotechnology

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U each
EUR 272.4

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20600 5000U
EUR 49

AtMax Taq DNA Polymerase (2.5U/µl), 200U

PL4201 each Ask for price

AtMax Taq DNA Polymerase (2.5U/µl), 500U

PL4202 each Ask for price

Chromo Taq DNA Polymerase (1U/µl), 200U

PL1205 each Ask for price

Chromo Taq DNA Polymerase (1U/µl), 500U

PL1206 each Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U each
EUR 126

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

Taq HS DNA Polymerase (Glycerol-free)

QL101-01 1,000 U
EUR 71.2

Taq HS DNA Polymerase (Glycerol-free)

QL101-02 5,000 U
EUR 324.8

Taq HS DNA Polymerase (Glycerol-free)

QL101-03 50,000 U
EUR 2900

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 710.4

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 276

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 393.6

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1388.4

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 6540

Taq DNA Polymerase M101 (5,000 U/mL)

RK26000 250U
EUR 60

DNA Amplification Kit with Taq DNA Polymerase, 100app

PL1202-K each Ask for price

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 896.24

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Taq DNA Polymerase (High Purity), 5u/ul

HTD0078 5X200U
EUR 129.6

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

ACTaq™ Taq Blue DNA Polymerase, 1000U

E2100B 1000U
EUR 153.24

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 157.2

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 230.4

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 375.6

Genorise® CLT Taq DNA Polymerase (1000 U)

GR108003 1000 U
EUR 199

Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled

AD-146-F Custom Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-1 500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-2 2500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-3 5000 Unit (s) Ask for price

Cheetah™ Hot Start Taq DNA Polymerase

29050-1 EA
EUR 277

HS Taq DNA Polymerase M101 (5,000 U /mL)

RK26201 1000U
EUR 39

DNA Amplification Kit with Max Taq DNA Polymerase, 100app

PL2202-K each Ask for price

Taq DNA Polymerase 2x Premix - 500 Reactions

3249 1/EA
EUR 228

Taq DNA Polymerase 2x Premix - 1000 Reactions

3250 1000
EUR 255
Description: Intact Genomics Taq DNA Polymerase 2x Premix with Dye is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. Intact Genomics Taq DNA Polymerase 2x Premix with Dye is ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, and stabilizers. It has been optimized for routine PCR applications.Product Includes:Taq DNA Polymerase 2x Premix with Dye5x Magic Enhancer

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 196.8

DNA Amplification Kit with Chromo Taq DNA Polymerase, 100app

PL1206-K each Ask for price

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-001 1000 units
EUR 198

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-005 5000 units
EUR 360

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-010 10,000 units
EUR 699.6

Taq DNA Polymerase, Ultra Pure, 5 units/ul

T2016-100 100,000 units
EUR 3462

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 490.8

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 420.65

Taq DNA Polymerase - 1000 units with separate dNTP Mix

3243d 1000units
EUR 150
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

DNA Amplification Kit with Chromo Max Taq DNA Polymerase, 100app

PL2206-K each Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950 - Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950L - Ask for price

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

Taq Polymerase

PCR-211L 5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-211S 200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli

Taq Polymerase

PCR-420-10KU 10kilo units
EUR 1043.5

Several molecules have been shown interesting activity against murine myeloid leukemia virus reverse transcriptase (MMLV-RT) (compounds 20 and 28 were produced inhibition with IC50 values ​​of 38.62 and 50.98 pM, respectively) and Taq DNA polymerase (analog 13 and 14 are produced inhibition with IC50 values ​​of 48.08 and 57.88 pM, respectively). The inhibitor may have an interest as antiretroviral chemotherapeutic agents and also in the development of anticancer drugs.