Description: This product includes 300 ul of 10 x assay buffer, 200 ul of 10 x concatenated DNA (30 ug/ml), 200 ul of 10 x ATP, 200 ul of 0.5 M EDTA, 15 ul of 200 x human topoisomerase II alpha (10 U/µl), 1 ml of enzyme dilution buffer and 500 ul of 5 x gel-loading buffer for 100 assays of human DNA decatenation reactions.

Description: The PD-1/PD-L1 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L1 interaction. The assay consists of two main components:_x000D_• Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment._x000D_• Expression vectors for TCR activator, and Human PD-L1: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L1 and an engineered cell surface T cell receptor (TCR) activator.

Description: The PD-1/PD-L2 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L2 interaction. The assay consists of two main components:_x000D_• Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing Human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment._x000D_• Expression vectors for TCR activator and Human PD-L2: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L2 and an engineered cell surface T cell receptor (TCR) activator.

Description: This product includes all the reagents for 100 samples in gel-based assays of E. coli topoisomerase IV DNA decatenation activity. It includes 300 µl of 10 x Buffer, 205 µl of 60 mg/ml concatenated DNA (kDNA), 205 µl of 10 mM ATP, 550 µl of 5 x Gel loading buffer and 22 µl of 10 µM E.coli topoisomerase IV (the parC-parE complex).

Description: The PD-1/PD-L1 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of PD-1:PD-L1 or PD-1:PD-L2 interaction. The assay consists of two main components:_x000D_ • PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing human PD-1. These cryopreserved cells are provided in a thaw-and-use format that does not require cell propagation._x000D_ • _x000D_Expression vectors for TCR activator, human PD-L1, and human PD-L2: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L1 or PD-L2 and an engineered cell surface T cell receptor (TCR) activator._x000D_